Hayashi M, Maruo T, Matsuo H, Mochizuki M
Nihon Naibunpi Gakkai Zasshi. 1985 Oct 20;61(10):1189-96. doi: 10.1507/endocrine1927.61.10_1189.
To elucidate if the thyroid hormone acts directly on the ovary, the biocellular effect of L-thyroxine (T4) on porcine granulosa cells cultured in vitro was investigated. Monolayer cultures of porcine granulosa cells obtained from small (1 approximately 2 mm), medium (3 approximately 5 mm) or large (6 approximately 11 mm) follicles were carried out in the presence of porcine FSH (100 ng/ml). Concomitant treatment with T4 promoted FSH-dependent morphological luteinization, i.e. alteration of immature granulosa cells obtained from small follicles to epithelioid form. T4 also increased FSH-stimulated induction of hCG/LH receptor on immature granulosa cells. Furthermore, T4 augmented FSH-mediated production of progesterone and estradiol by immature granulosa cells cultured in vitro. The concentration of T4 to produce the maximal stimulatory effect was 10-7 M, demonstrating that optimal concentration of thyroid hormone is required for the expression of this stimulatory action. Since T4 alone demonstrated no effect on the differentiation of porcine granulosa cells and all the stimulatory effect of T4 seems to have a permissive action on FSH-induced granulosa cell luteinization. Although insulin showed a similar effect on porcine granulosa cells, no stimulation of estradiol production by porcine granulosa cells was observed with insulin in the culture system used in this study. These results suggest that the thyroid hormone acts directly on the ovary and plays an important role in modifying the FSH-dependent cellular differentiation of immature granulosa cells.
为了阐明甲状腺激素是否直接作用于卵巢，研究了L-甲状腺素（T4）对体外培养的猪颗粒细胞的生物细胞效应。在猪促卵泡素（FSH，100 ng/ml）存在的情况下，对从小（1至2 mm）、中（3至5 mm）或大（6至11 mm）卵泡获得的猪颗粒细胞进行单层培养。T4的联合处理促进了FSH依赖性形态黄体化，即从小卵泡获得的未成熟颗粒细胞转变为上皮样形态。T4还增加了FSH刺激的未成熟颗粒细胞上hCG/LH受体的诱导。此外，T4增强了体外培养的未成熟颗粒细胞中FSH介导的孕酮和雌二醇的产生。产生最大刺激作用的T4浓度为10-7 M，表明这种刺激作用的表达需要最佳浓度的甲状腺激素。由于单独的T4对猪颗粒细胞的分化没有影响，T4的所有刺激作用似乎对FSH诱导的颗粒细胞黄体化具有允许作用。尽管胰岛素对猪颗粒细胞显示出类似的作用，但在本研究使用的培养系统中，未观察到胰岛素对猪颗粒细胞产生雌二醇的刺激作用。这些结果表明，甲状腺激素直接作用于卵巢，在调节未成熟颗粒细胞的FSH依赖性细胞分化中起重要作用。
