Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Dec 5;205:292-297. doi: 10.1016/j.saa.2018.07.040. Epub 2018 Jul 20.
Women are the most ones who susceptible to a common syndrome called fibromyalgia syndrome, up to 90% of all people with fibromyalgia are women. It affects mainly muscles and soft tissue and cause for them muscle pain, sleep problems and painful tender points. Milnacipran is acting as a serotonin-norepinephrine reuptake inhibitor (SNRI) therefore, it is recommended for the treatment of this syndrome. The widespread use of this compound in our market requires the development and validation of a simple, sensitive, cheap, fast and reproducible spectrofluorimetric method for the assay of milnacipran hydrochloride in its pure state, pharmaceutical tablets and spiked human urine and plasma. In the current work ninhydrin and phenylacetaldehyde in Teorell and Stenhagen buffer (pH 7) reacts with the amino moiety of milnacipran through a sensitive condensation reaction resulting in formation of a fluorescent product, which exhibits its fluorescence emission intensity at 465 nm after excitation at 390 nm. It is observed that, in the concentration range 0.5 to 3.0 μgmL, the constructed calibration curve was linear with a good correlation coefficient (0.9998). The condensation reaction was successfully applied for the assay of the studied drug in Avermilan® tablets, spiked human urine and plasma without interference from the components of the sample matrix with average percentage recoveries of 101.73 ± 0.56 and 100.55 ± 0.64 for urine and plasma, respectively.
女性是最容易患上一种名为纤维肌痛综合征的常见综合征的人群，高达 90%的纤维肌痛综合征患者都是女性。它主要影响肌肉和软组织，导致肌肉疼痛、睡眠问题和痛点压痛。米那普仑作为一种 5-羟色胺和去甲肾上腺素再摄取抑制剂（SNRI），因此被推荐用于治疗这种综合征。由于这种化合物在我们市场上的广泛应用，需要开发和验证一种简单、灵敏、廉价、快速且可重现的分光荧光法，用于测定米那普仑盐酸盐在其纯态、药物片剂和加标人尿和血浆中的含量。在当前的工作中，萘啶酮和苯乙酮在 Teorell 和 Stenhagen 缓冲液（pH 7）中与米那普仑的氨基部分通过灵敏的缩合反应反应，形成荧光产物，在 390nm 激发后，其荧光发射强度在 465nm 处。结果表明，在 0.5 至 3.0μgmL 的浓度范围内，构建的校准曲线呈线性，相关系数良好（0.9998）。该缩合反应成功地应用于 Avermilan®片剂、加标人尿和血浆中研究药物的测定，样品基质成分无干扰，尿和血浆的平均百分回收率分别为 101.73±0.56 和 100.55±0.64。
