比较分析链霉菌基因组序列中的 CRISPR 基因座。
Comparative Analysis of CRISPR Loci Found in Streptomyces Genome Sequences.
机构信息
State Key Laboratory of Microbial Metabolism, Joint International Laboratory on Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
出版信息
Interdiscip Sci. 2018 Dec;10(4):848-853. doi: 10.1007/s12539-018-0301-8. Epub 2018 Jul 20.
The interspaced short palindromic repeats (CRISPR) system is an immune system widely distributed in prokaryotes, resisting the invasion of the foreign mobile genetic elements like phages or plasmids. In this study, we present the comparative analysis of 182 CRISPR loci found in 46 publicly available complete genome sequences of Streptomyces. Overall, nine direct repeats (DRs) groups are identified while all the 2104 spacers are divided into three main groups according to the multiple sequence alignment. Only 11 spacers are identical with parts of 10 plasmid sequences, which indicates a possible origin. The cas gene clusters near the CRISPR arrays are found to mainly belong to the I-E subtype. These CRISPR loci might play an important role in the genome evolution of Streptomyces.
间隔短回文重复序列（CRISPR）系统是一种广泛分布于原核生物中的免疫系统，可抵抗噬菌体或质粒等外来移动遗传元件的入侵。在这项研究中，我们对 46 个公开提供的链霉菌完整基因组序列中的 182 个 CRISPR 基因座进行了比较分析。总的来说，我们确定了 9 个直接重复（DR）簇，而根据多序列比对，2104 个间隔区则分为三个主要组。只有 11 个间隔区与 10 个质粒序列的部分序列相同，这表明它们可能有一个共同的起源。我们发现，CRISPR 基因座附近的 cas 基因簇主要属于 I-E 亚型。这些 CRISPR 基因座可能在链霉菌的基因组进化中发挥了重要作用。
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