State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Department of Food Science, Cornell University, Ithaca, NY 14850, USA.
Department of Food Science, Cornell University, Ithaca, NY 14850, USA.
Int J Food Microbiol. 2018 Nov 20;285:61-68. doi: 10.1016/j.ijfoodmicro.2018.07.010. Epub 2018 Jul 10.
The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing. The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage. Using high-throughput sequencing analysis, the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes and the bacteria family of Lachnospiraceae and Bacteroidaceae were observed in fresh grass carp. As storage time increased, the coated samples retained bacterial diversity. However, Shewanellaceae, Pseudomonadaceae and Flavobacteriaceae increased and became the predominant microbiota in spoiled control samples. The significant difference between the bacteria species in the control and coated samples showed that the coating had the potential to inhibit microbial growth, especially spoilage microorganisms, and reduced quality deterioration caused by bacteria during refrigerated storage of grass carp fillets.
在冷藏 15 天期间，研究了基于壳聚糖的涂层对草鱼（Ctenopharyngodon idellus）鱼片微生物腐败抑制的影响，从 pH 值、总挥发性碱基氮（TVB-N）、三甲胺（TMA）、三磷酸腺苷（ATP）相关化合物、K 值、微生物计数和高通量测序方面进行了评估。结果表明，用含有 0.3%单月桂酸甘油酯和 0.5%丁香精油的壳聚糖基涂层处理的鱼片，其 TVB-N、TMA、次黄嘌呤核苷（HxR）、次黄嘌呤（Hx）和 K 值较低，与对照相比，在第 15 天的冷藏中，分别显著降低了近 34%、73%、32%、74%和 38%（P<0.05）。通过高通量测序分析，在新鲜草鱼中观察到厚壁菌门、蓝藻门和拟杆菌门等主要细菌门，以及lachnospiraceae 和 bacteroidaceae 等细菌科。随着储存时间的增加，涂层样品保持了细菌多样性。然而，Shewanellaceae、Pseudomonadaceae 和 Flavobacteriaceae 增加，并成为变质对照样品中的主要微生物群。对照和涂层样品中细菌种类的显著差异表明，该涂层具有抑制微生物生长的潜力，特别是腐败微生物，并减少了冷藏草鱼鱼片储存过程中细菌引起的质量恶化。
