Teutsch H F
J Histochem Cytochem. 1986 Feb;34(2):263-7. doi: 10.1177/34.2.3003183.
In conjunction with the investigation of intercellular compartmentation of liver carbohydrate metabolism, a new procedure for isolation of tissue samples from freeze-dried cryosections was developed. It was designed to permit assessment of functional differences between sinusoids of portal and septal origin, and to extend investigation of liver cell heterogeneity along sinusoids to the level of the structural-functional unit. Application of this procedure, together with microchemical assays of high analytical sensitivity, enabled measurement of 50 individual glucose and glucose-6-P values in a single cross-sectional area of about 0.75 mm2 of a liver unit. Preliminary results on the distribution of glucose and glucose-6-P indicated that, in a state of overall glucose release glucose levels were significantly higher in the center than in the periphery of the unit. Overall glucose release by the liver resulted from both release and uptake of glucose along sinusoids. Glucose-6-P was highest in the periphery and decreased toward the center. Microchemical data, furthermore, indicated possible functional heterogeneity of sinusoids, insofar as both glucose and glucose-6-P gradients were steeper in "portal-central" than in "septal-central" sinusoids.
在对肝脏碳水化合物代谢的细胞间分隔进行研究的过程中，开发了一种从冷冻干燥的冰冻切片中分离组织样本的新方法。该方法旨在评估门静脉窦和间隔窦之间的功能差异，并将沿窦状隙的肝细胞异质性研究扩展到结构 - 功能单元水平。此方法与高分析灵敏度的微量化学分析相结合，能够在约0.75平方毫米的肝脏单元单个横截面积中测量50个个体的葡萄糖和6 - 磷酸葡萄糖值。关于葡萄糖和6 - 磷酸葡萄糖分布的初步结果表明，在整体葡萄糖释放状态下，单元中心的葡萄糖水平显著高于周边。肝脏的整体葡萄糖释放是沿窦状隙的葡萄糖释放和摄取共同作用的结果。6 - 磷酸葡萄糖在周边最高，并向中心降低。此外，微量化学数据表明窦状隙可能存在功能异质性，因为在“门静脉 - 中心”窦状隙中葡萄糖和6 - 磷酸葡萄糖梯度比“间隔 - 中心”窦状隙中更陡。
