Singh Sanjesh, Grice I Darren, Peak Ian R, Frost Thomas, Yue Geng, Wilson Jennifer C
Menzies Health Institute Queensland, Griffith University, Gold Coast, Australia; Institute for Glycomics, Griffith University, Gold Coast, Australia; School of Medical Science, Griffith University, Gold Coast, Australia.
Institute for Glycomics, Griffith University, Gold Coast, Australia; School of Medical Science, Griffith University, Gold Coast, Australia.
Carbohydr Res. 2018 Sep;467:1-7. doi: 10.1016/j.carres.2018.07.002. Epub 2018 Jul 20.
The Gram-negative bovine pathogen Moraxella bovis is a causative agent of Infectious bovine keratoconjunctivitis, 'pink-eye' that affects cattle. Here we report that strain L183/2 has the same capsular polysaccharide (CPS) of unsulfated chondroitin, as does strain Mb25, whereas strain Epp63 does not express CPS. NMR analysis of the oligosaccharides (OS) derived from the lipooligosaccharides (LOS) in these three strains by NMR has shown that strain Mb25 and Epp63 have the same OS structure with a terminal N-acetylgalactosamine ((1S)-GalaNAc) residue →4,6-linked. Strain L183/2 lacks the (1 S)-GalaNAc residue. The biological role of M. bovis LOS was assessed by comparing the LOS from strains Epp63, Mb25 and L183/2 and truncated Epp63 LOS variants. LOS truncation affected M. bovis growth rate, susceptibility to antibiotics, detergents, bovine serum bactericidal activity, endotoxicity and adherence to HeLa cells.
革兰氏阴性牛病原体牛莫拉菌是感染性牛角膜结膜炎（即影响牛的“红眼病”）的病原体。在此我们报告，L183/2菌株与Mb25菌株具有相同的未硫酸化软骨素荚膜多糖（CPS），而Epp63菌株不表达CPS。通过核磁共振（NMR）对这三种菌株脂寡糖（LOS）衍生的寡糖（OS）进行分析表明，Mb25菌株和Epp63菌株具有相同的OS结构，带有一个末端N - 乙酰半乳糖胺（(1S)-GalaNAc）残基，以→4,6 - 连接。L183/2菌株缺少(1S)-GalaNAc残基。通过比较Epp63、Mb25和L183/2菌株以及截短的Epp63 LOS变体的LOS，评估了牛莫拉菌LOS的生物学作用。LOS截短影响了牛莫拉菌的生长速率、对抗生素和去污剂的敏感性、牛血清杀菌活性、内毒素活性以及对HeLa细胞的黏附。
