College of Horticulture, Fujian Agriculture and Forestry University, Key Laboratory of Tea Science in Universities of Fujian Province, Fuzhou, China.
College of Horticulture, Fujian Agriculture and Forestry University, Key Laboratory of Tea Science in Universities of Fujian Province, Fuzhou, China.
J Plant Physiol. 2018 Oct;229:41-52. doi: 10.1016/j.jplph.2018.07.002. Epub 2018 Jul 17.
Trichome (also referred to as 'háo' in tea) is a key feature in both tea products and tea plant (Camellia sinensis) selection breeding. Although trichomes are used as a model for studying cell differentiation and have been well studied in many plant species, the regulation of trichome formation at the molecular level is poorly understood in tea plants. In the present study, the hairy and hairless tea plant cultivars Fudingdabaicha (FDDB) and Rongchunzao (RCZ), respectively, were used to study this mechanism. We characterised tea plant trichomes as unicellular and unbranched structures. High-throughput Illumina sequencing yielded approximately 277.0 million high-quality clean reads from the FDDB and RCZ cultivars. After de novo assembly, 161,444 unigenes were generated, with an average length of 937 bp. Among these unigenes, 81,425 were annotated using public databases, and 55,201 coding sequences and 4004 transcription factors (TFs) were identified. In total, 21,599 differentially expressed genes (DEGs) were identified between RCZ and FDDB, of which 10,785 DEGs were up-regulated and 10,814 DEGs were down-regulated. Genes involved in the DNA replication pathway were significantly enriched. Furthermore, between FDDB and RCZ, DEGs related to TFs, phytohormone signals, and cellulose synthesis were identified, suggesting that certain genes involved in these pathways are crucial for trichome initiation in tea plants. Together, the results of this study provide novel data to improve our understanding of the potential molecular mechanisms of trichome formation and lay a foundation for additional trichome studies in tea plants.
茸毛（在茶中也被称为“毫”）是茶叶产品和茶树选择育种的一个关键特征。虽然茸毛被用作研究细胞分化的模型，并且在许多植物物种中得到了很好的研究，但在茶树中，茸毛形成的分子调控机制还知之甚少。本研究分别使用有毛和无毛的茶树品种福鼎大白茶（FDDB）和荣春早（RCZ）来研究这一机制。我们将茶树茸毛描述为单细胞和不分枝的结构。高通量 Illumina 测序从 FDDB 和 RCZ 品种中获得了约 2.77 亿条高质量清洁读数。经过从头组装，共生成了 161444 条 unigenes，平均长度为 937bp。在这些 unigenes中，有 81425 条使用公共数据库进行了注释，鉴定出了 55201 条编码序列和 4004 个转录因子（TFs）。总共在 RCZ 和 FDDB 之间鉴定出了 21599 个差异表达基因（DEGs），其中 10785 个上调，10814 个下调。参与 DNA 复制途径的基因显著富集。此外，在 FDDB 和 RCZ 之间，还鉴定出了与 TFs、植物激素信号和纤维素合成相关的 DEGs，这表明这些途径中的某些基因对茶树茸毛的起始至关重要。总之，本研究的结果提供了新的数据，有助于提高我们对茶树茸毛形成潜在分子机制的理解，并为茶树茸毛的进一步研究奠定了基础。
