Department of Chemical Engineering, Indian Institute of Technology Kharagpur, West Bengal 721302, India.
Soft Matter. 2018 Sep 19;14(36):7335-7346. doi: 10.1039/c8sm01146j.
Microscopic investigations of any abnormality associated with erythrocyte/red blood cell morphology constitute an important segment of the age-old peripheral smear test. Though the test is conducted on a glass slide, the effect of glass and similar other solid substrates on erythrocyte morphology remained majorly unexplored. In the first of its kind investigation, we have outlined the effect of varying the substrate surface potential on erythrocyte morphology. Such a substrate induced phenomenon has been quantified for two distinctly different drying configurations (droplets and film) upon systematically varying the cell concentration. Experimental results and supporting theoretical analysis unambiguously show the surface potential of the solid substrate to be the most influential parameter in the process of morphological alteration. The findings of the present investigation may be utilized to formulate an error-free protocol for the baseline peripheral smear test of hematological diagnosis.
对与红细胞/红血球形态相关的任何异常的微观研究构成了古老的外周涂片测试的一个重要部分。尽管该测试是在玻璃载玻片上进行的，但玻璃和类似的其他固体基质对红细胞形态的影响在很大程度上仍未得到探索。在首次此类调查中，我们概述了改变基底表面电势对红细胞形态的影响。对于两种明显不同的干燥配置（液滴和薄膜），通过系统地改变细胞浓度，对这种基底诱导的现象进行了量化。实验结果和支持的理论分析明确表明，固体基底的表面电势是形态改变过程中最具影响力的参数。本研究的结果可用于制定无错误的血液学诊断外周血涂片测试的标准方案。
