Adelantado Carlos, Murtada Khaled, Ríos Ángel, Zougagh Mohammed
Analytical Chemistry & Food Technology Department, University of Castilla-La Mancha, Camilo José Cela Avenue, E-13005, Ciudad Real, Spain.
Regional Institute for Applied Science Research, IRICA, Camilo José Cela Avenue, E-13005, Ciudad Real, Spain.
Bioanalysis. 2018 Aug 1;10(15):1193-1205. doi: 10.4155/bio-2018-0045. Epub 2018 Jul 23.
The preparation of magnetic multi-walled carbon nanotube poly(styrene-co-divinylbenzene) for propranolol magnetic solid-phase extraction is described.
MATERIALS & METHODS: A study comparing propranolol adsorption and desorption was performed with only magnetic multi-walled carbon nanotubes, and different poly(styrene-co-divinylbenzene) with and without magnetic multi-walled carbon nanotubes. Enantiomeric separation of propranolol took place by cyclodextrin-modified capillary electrophoresis and the method was validated in spiked human urine samples.
Recovery values raised when styrene/divinylbenzene millimoles ratio was 19.57:15.80. Enrichment factors increased up to approximately 100, detection limits were 13.8 and 10.5 ng ml for R- and S-propranolol respectively, quantitation limits were 46.0 and 34.8 ng ml for R- and S-propranolol respectively, recoveries from spiked samples ranged from 90.9 to 109.0%, and relative standard deviations were <6.3%.
This methodology was proven to be more effective than classical solid-phase extraction strategies and may be applied to other kind of biological samples.
描述用于普萘洛尔磁性固相萃取的磁性多壁碳纳米管聚（苯乙烯 - 共 - 二乙烯基苯）的制备。
使用仅磁性多壁碳纳米管以及有无磁性多壁碳纳米管的不同聚（苯乙烯 - 共 - 二乙烯基苯）进行普萘洛尔吸附和解吸的比较研究。通过环糊精修饰的毛细管电泳进行普萘洛尔的对映体分离，并在加标的人尿样中验证该方法。
当苯乙烯/二乙烯基苯毫摩尔比为19.57:15.80时，回收率提高。富集因子增加至约100，R - 和S - 普萘洛尔的检测限分别为13.8和10.5 ng/ml，R - 和S - 普萘洛尔的定量限分别为46.0和34.8 ng/ml，加标样品的回收率范围为90.9%至109.0%，相对标准偏差<6.3%。
该方法被证明比经典固相萃取策略更有效，可应用于其他类型的生物样品。
