Struyker-Boudier H A, Vervoort-Peters L H, Rousch M J, Smits J F, Thijssen H H
Life Sci. 1986 Jan 13;38(2):137-45. doi: 10.1016/0024-3205(86)90005-6.
Beta-adrenoceptor binding characteristics were determined in different fractions of rat kidney tubules using a [125Iodo]-(-)-cyanopindolol (ICYP) binding assay. The highest amount of binding sites was found in a fraction containing predominantly distal tubular fragments. In a separate series of experiments the ICYP binding characteristics were compared in whole tubular fractions from spontaneously hypertensive (SHR) and normotensive Wistar Kyoto rats (WKY) of different ages. The maximum number of binding sites was significantly higher both in young (3 weeks) and adult (14 weeks) SHR when compared to age-matched WKY. These studies showed the presence of beta-adrenoceptor binding sites in rat kidney tubules and support the potential importance of tubular beta-adrenoceptors in the development of spontaneous hypertension and in the mechanism of antihypertensive action of beta-blockers.
采用[125I标记]-（-）-氰基吲哚洛尔（ICYP）结合试验，测定大鼠肾小管不同组分中的β-肾上腺素能受体结合特性。在主要含有远端肾小管片段的组分中发现了最高数量的结合位点。在另一系列实验中，比较了不同年龄的自发性高血压大鼠（SHR）和正常血压的Wistar Kyoto大鼠（WKY）全肾小管组分中的ICYP结合特性。与年龄匹配的WKY相比，年轻（3周）和成年（14周）SHR的最大结合位点数均显著更高。这些研究表明大鼠肾小管中存在β-肾上腺素能受体结合位点，并支持肾小管β-肾上腺素能受体在自发性高血压发生发展及β受体阻滞剂降压作用机制中的潜在重要性。
