Nakai T, Oida K, Tamai T, Kutsumi Y, Miyabo S, Takeda R
Life Sci. 1986 Feb 17;38(7):653-62. doi: 10.1016/0024-3205(86)90059-7.
Characteristics of lipoprotein receptors of the isolated liver parenchymal cells prepared from the streptozotocin-induced diabetic rats were investigated. Streptozotocin-induced diabetic rats fed 1.0% cholesterol showed the exaggerated hypercholesterolemia as compared to control rats fed 1.0% cholesterol. The present study was designed to elucidate the role of lipoprotein receptor mechanisms of liver parenchymal cells in the diabetic dyslipoproteinemia. 125I-labeled lipoproteins (rat beta-VLDL, human LDL2 or rat HDL3) were incubated with liver parenchymal cells isolated by liver perfusion using collagenase. According to the Scatchard analysis, the apparent dissociation constant (kd) and maximum beta-VLDL binding (Bmax) for the higher affinity binding site in the diabetic rats (n = 6) were (11.9 +/- 5.1) X 10(2) ng/ml and 307.5 +/- 145.2 ng/10(6) cells, respectively. These binding characteristics of the diabetic rats were not significantly different from the control rats. Furthermore, there were no significant differences in the binding characteristics of human LDL2 and rat HDL3 between the diabetic rats and the control rats. The data presented suggest that significant role of alteration of lipoprotein receptor characteristics in liver parenchymal cells is not played in the diabetic dyslipoproteinemia.
研究了从链脲佐菌素诱导的糖尿病大鼠分离的肝实质细胞脂蛋白受体的特性。与喂食1.0%胆固醇的对照大鼠相比，喂食1.0%胆固醇的链脲佐菌素诱导的糖尿病大鼠出现了更严重的高胆固醇血症。本研究旨在阐明肝实质细胞脂蛋白受体机制在糖尿病血脂异常中的作用。用胶原酶通过肝脏灌注分离的肝实质细胞与125I标记的脂蛋白（大鼠β-VLDL、人LDL2或大鼠HDL3）一起孵育。根据Scatchard分析，糖尿病大鼠（n = 6）中高亲和力结合位点的表观解离常数（kd）和最大β-VLDL结合量（Bmax）分别为（11.9±5.1）×10（2）ng/ml和307.5±145.2 ng/10（6）个细胞。糖尿病大鼠的这些结合特性与对照大鼠无显著差异。此外，糖尿病大鼠与人LDL2和大鼠HDL3的结合特性与对照大鼠之间也无显著差异。所呈现的数据表明，肝实质细胞脂蛋白受体特性的改变在糖尿病血脂异常中没有发挥重要作用。
