Shi Xiao-feng, Meng Chen, Ma Li-zhen, Ma Hai-kuan, Zhang Xin-min, Ma Jun
Guang Pu Xue Yu Guang Pu Fen Xi. 2016 Jul;36(7):2128-33.
In this paper, Au nanosphere and Au-Ag alloy nanourchin were prepared by reducing the chloroauric acid. The mixed colloid solutions of Au nanosphere and Au-Ag alloy nanourchin were used as surface-enhanced Raman scattering (SERS) substrate to detect polycyclic aromatic hydrocarbons (PAHs) in aqueous solution. The size of Au-Ag alloy nanourchin particle was about 300400 nm and the thorn-like bulge covered on it was about 40100 nm. The mixed colloid solutions of Au nanosphere and Au-Ag alloy nanourchin which were optimized pH values and other parameters presented a better enhancement than Au nanosphere. The enhancement effect was about three times that of Au nanosphere colloid solution. Three kinds of high molecular weight PAHs, pyrene(4 rings), benzoanthracene(4 rings) and benzo[a]pyrene(5 rings), were detected. The results showed that there were good linear relationships between Raman intensity and concentration in the low concentration range and the mixed SERS substrate had a good reproducibility and stability. Their limits of detection (LODs) were 0.44, 2.92 and 1.64 nmol·L-1, respectively. The innovation of this paper was that the mixed colloid solutions of Au nanosphere and Au-Ag alloy nanourchin are prepared as SERS substrate and the trace-level high molecular weight PAHs are detected. The results show that the detection of trace-level high molecular weight PAHs in aqueous can be realized using the mixed SERS substrate prepared in this study, which proposed an in-situ method for detecting the high molecular weight PAHs in aqueous.
本文通过氯金酸还原法制备了金纳米球和金 - 银合金纳米海胆。将金纳米球与金 - 银合金纳米海胆的混合胶体溶液用作表面增强拉曼散射（SERS）基底，以检测水溶液中的多环芳烃（PAHs）。金 - 银合金纳米海胆颗粒尺寸约为300400 nm，其表面覆盖的刺状凸起约为40100 nm。优化pH值等参数后的金纳米球与金 - 银合金纳米海胆混合胶体溶液比金纳米球表现出更好的增强效果。增强效果约为金纳米球胶体溶液的三倍。检测了三种高分子量PAHs，芘（4环）、苯并蒽（4环）和苯并[a]芘（5环）。结果表明，在低浓度范围内拉曼强度与浓度之间存在良好的线性关系，混合SERS基底具有良好的重现性和稳定性。它们的检测限（LODs）分别为0.44、2.92和1.64 nmol·L-1。本文的创新之处在于制备了金纳米球与金 - 银合金纳米海胆的混合胶体溶液作为SERS基底并检测痕量水平的高分子量PAHs。结果表明，利用本研究制备的混合SERS基底可实现对水溶液中痕量高分子量PAHs的检测，为水溶液中高分子量PAHs的检测提出了一种原位方法。
