辛伐他汀对紫外线 B 诱导的角膜内皮细胞死亡的保护作用。

The protective effect of simvastatin against ultraviolet B-induced corneal endothelial cell death.

机构信息

Department of Medical Research and Development, Chang Bing Show Chwan Memorial Hospital, Changhua, Taiwan.

Department of Internal Medicine, Cathay General Hospital, Taipei, Taiwan.

出版信息

Indian J Ophthalmol. 2018 Aug;66(8):1080-1083. doi: 10.4103/ijo.IJO_93_18.

DOI:10.4103/ijo.IJO_93_18

PMID:30038146

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6080451/

Abstract

PURPOSE

Excessive ultraviolet B (UVB) exposure causing corneal endothelium injury, including apoptosis, is a serious condition. Therefore, drugs that can inhibit apoptosis in corneal endothelial cells represent an effective strategy. Simvastatin is widely used as a specific inhibitor of 3-hydroxy-3-methyl-glutaryl-CoA reductase, can reduce levels of low density lipoprotein (LDL) cholesterol, and exerts anti-inflammatory effects. However, the protective effect of simvastatin on corneal endothelial cells remains unclear. Therefore, the aim of this study was to elucidate whether UVB promotes the initiation of apoptosis in corneal endothelial cells and injury reversible by simvastatin treatment.

METHODS

We detected the cell viability, subG1 population, and caspase-3 activity.

RESULTS

Results showed that simvastatin alleviates UVB-induced cell death, cell apoptosis, and caspase-3 activity.

CONCLUSION

Our findings indicated that simvastatin alleviated UVB-induced corneal endothelial cell apoptosis via caspase-3 activity.

摘要

目的

过量的紫外线 B（UVB）照射会导致角膜内皮细胞损伤，包括细胞凋亡，这是一种严重的情况。因此，能够抑制角膜内皮细胞凋亡的药物代表了一种有效的策略。辛伐他汀被广泛用作 3-羟基-3-甲基戊二酰辅酶 A 还原酶的特异性抑制剂，可降低低密度脂蛋白（LDL）胆固醇水平，并发挥抗炎作用。然而，辛伐他汀对角膜内皮细胞的保护作用尚不清楚。因此，本研究旨在阐明 UVB 是否会促进角膜内皮细胞凋亡的发生，以及辛伐他汀治疗是否可以逆转这种损伤。