Ophthalmology Department, KaplanMedical Center, Rehovot, Israel.
J Cataract Refract Surg. 2010 Nov;36(11):1972-5. doi: 10.1016/j.jcrs.2010.05.023.
To evaluate the protective effect of different ophthalmic viscosurgical devices on corneal endothelial cells during phacoemulsification in a rabbit model.
Harlan Biotech Israel and Ophthalmology Department, Kaplan Medical Center, Rehovot, Israel.
Experimental study.
Rabbit eyes were randomly assigned to 3 equally sized groups. Endothelial cell counts were performed in all eyes before initiation of the study. The aqueous humor was completely replaced by Biolon (sodium hyaluronate 1.0%) in Group A, by a combination of Viscoat (sodium chondroitin sulfate 4.0%-sodium hyaluronate 3.0%) and Provisc (sodium chondroitin sulfate 1.0%) using the soft-shell technique in Group B, and by a combination of Visiol (sodium hyaluronate 2.0%-mannitol 0.5%) and Biolon using the soft-shell technique in Group C. The eyes were exposed to alternating 10 seconds of phacoemulsification and a 10-second pause until a total exposure time of 2.5 minutes elapsed. Endothelial cell counts were repeated 3 days after surgery.
The study used 18 rabbit eyes, 6 in each group. Group A had the highest endothelial cell loss (13%) followed by Group B (7%), and Group C (4%). The difference in cell loss between Group C and Group A was statistically significant (P = .037).
The study showed the efficiency and advantages of the soft-shell technique using the combination of Visiol and Biolon over Biolon alone.
在兔模型中评估不同眼科黏弹剂在超声乳化过程中对角膜内皮细胞的保护作用。
以色列哈兰生物技术公司和以色列雷霍沃特卡普兰医学中心眼科部。
实验研究。
将兔眼随机分为 3 组，每组大小相等。所有眼睛在研究开始前均行内皮细胞计数。A 组兔眼房水完全被 Biolon（透明质酸钠 1.0%）取代，B 组兔眼采用软壳技术，以 Viscoat（硫酸软骨素钠 4.0%-透明质酸钠 3.0%）和 Provisc（硫酸软骨素钠 1.0%）的混合物取代房水，C 组兔眼采用软壳技术，以 Visiol（透明质酸钠 2.0%-甘露醇 0.5%）和 Biolon 的混合物取代房水。暴露于超声乳化 10 秒，然后暂停 10 秒，直至总暴露时间达到 2.5 分钟。术后 3 天重复内皮细胞计数。
本研究共使用 18 只兔眼，每组 6 只。A 组内皮细胞丢失率最高（13%），其次是 B 组（7%）和 C 组（4%）。C 组与 A 组之间的细胞丢失差异有统计学意义（P =.037）。
本研究表明，采用 Visiol 和 Biolon 混合物的软壳技术比单独使用 Biolon 更有效、更有优势。
