Hudkova L V, Dehtiar R H, Chumachenko Iu V, Hulyi M F
Ukr Biokhim Zh. 1975 May-Jun;47(3):342-6.
A comparative study of properties (absorption spectra, thermostability, pH optimum, polyacrylamide gel electrophoresis, DEAE-cellulose separation) and structure (amino acid composition, finger-prints, carbohydrate composition) was performed for P. vitale catalase synthesized under different medium conditions. In all cases the results were similar. The only difference occured in the amount of synthesized proteins. A conclusion is drawn that under different nourishing conditions of the fungus the properties and structure of the catalase are unchanged, but the regulatory mechanisms of catalase and glucosooxidate synthesis undergo changes.
对在不同培养基条件下合成的维塔利毕赤酵母过氧化氢酶的性质(吸收光谱、热稳定性、最适pH值、聚丙烯酰胺凝胶电泳、二乙氨基乙基纤维素分离)和结构(氨基酸组成、指纹图谱、碳水化合物组成)进行了比较研究。在所有情况下,结果都是相似的。唯一的差异在于合成蛋白质的量。得出的结论是,在真菌的不同营养条件下,过氧化氢酶的性质和结构未发生变化,但过氧化氢酶和葡萄糖氧化酶合成的调节机制发生了变化。