Marine Life Science College, Ocean University of China, Qingdao 266003, China.
Marine Life Science College, Ocean University of China, Qingdao 266003, China.
Mar Pollut Bull. 2018 Aug;133:415-422. doi: 10.1016/j.marpolbul.2018.06.001. Epub 2018 Jun 19.
Estrogenic pollution has aroused great concern for its adverse effects on marine organisms. This study aimed to establish biomarker-based methods for detecting environmental estrogens using vitellogenin (Vtg) of teleost fishes inhabiting coastal areas of China. Firstly, Vtgs in marbled flounder (Pseudopleuronectes yokohamae), black rockfish (Sebastes schlegelii) and fat greenling (Hexagrammos otakii) were purified, characterized and used to prepare antibodies. Then, Vtg ELISA for each species was developed using purified Vtg and its antibody. Marbled flounder Vtg ELISA had a working range of 3.9-500 ng/mL and a detection limit of 2.1 ng/mL, and black rockfish Vtg ELISA had strong cross-reactivity with marbled flounder Vtg. Furthermore, Vtg induction in male marbled flounder exposed to pentadecafluorooctanoic acid (PFOA) was measured by developed ELISA. Plasma Vtg concentrations were significantly increased with PFOA concentrations in seawater and fish muscle. Therefore, Vtg ELISAs for these species might be useful tools for monitoring marine environmental estrogens.
雌激素污染因其对海洋生物的不良影响而引起了极大的关注。本研究旨在建立基于生物标志物的方法，使用中国沿海地区定居的鱼类卵黄蛋白原（Vtg）来检测环境雌激素。首先，从麻斑鲆（Pseudopleuronectes yokohamae）、青石斑鱼（Sebastes schlegelii）和高体绿鳍鱼（Hexagrammos otakii）中纯化、鉴定和制备卵黄蛋白原抗体。然后，使用纯化的 Vtg 及其抗体开发了针对每种鱼类的 Vtg ELISA。麻斑鲆 Vtg ELISA 的工作范围为 3.9-500ng/mL，检测限为 2.1ng/mL，并且对青石斑鱼 Vtg 具有很强的交叉反应性。此外，通过开发的 ELISA 测量了暴露于全氟辛酸（PFOA）的雄性麻斑鲆中的 Vtg 诱导。海水中和鱼肌肉中的 PFOA 浓度与血浆 Vtg 浓度呈显著正相关。因此，这些物种的 Vtg ELISA 可能是监测海洋环境雌激素的有用工具。
