The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA.
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.
Sci Rep. 2018 Jul 24;8(1):11162. doi: 10.1038/s41598-018-28727-w.
We previously investigated the transcriptome and proteome profiles of the murine ocular lens at six developmental time points including two embryonic (E15 and E18) and four postnatal time points (P0, P3, P6, and P9). Here, we extend our analyses to identify novel transcripts and peptides in developing mouse lens. We identified a total of 9,707 novel transcripts and 325 novel fusion genes in developing mouse lens. Additionally, we identified 13,281 novel alternative splicing (AS) events in mouse lens including 6,990 exon skipping (ES), 2,447 alternative 3' splice site (A3SS), 1,900 alternative 5' splice site (A5SS), 1,771 mutually exclusive exons (MXE), and 173 intron retention (IR). Finally, we integrated our OMIC (Transcriptome and Proteome) datasets identifying 20 novel peptides in mouse lens. All 20 peptides were validated through matching MS/MS spectra of synthetic peptides. To the best of our knowledge, this is the first report integrating OMIC datasets to identify novel peptides in developing murine lens.
我们之前研究了在六个发育时间点（包括两个胚胎期（E15 和 E18）和四个新生期（P0、P3、P6 和 P9））的鼠眼晶状体的转录组和蛋白质组图谱。在这里，我们扩展了我们的分析，以鉴定发育中的老鼠晶状体中的新转录本和肽。我们总共在发育中的小鼠晶状体中鉴定了 9707 个新转录本和 325 个新融合基因。此外，我们在小鼠晶状体中鉴定了 13281 个新的可变剪接（AS）事件，包括 6990 个外显子跳跃（ES）、2447 个替代 3'剪接位点（A3SS）、1900 个替代 5'剪接位点（A5SS）、1771 个互斥外显子（MXE）和 173 个内含子保留（IR）。最后，我们整合了我们的 OMIC（转录组和蛋白质组）数据集，在小鼠晶状体中鉴定了 20 个新的肽。所有 20 个肽都通过与合成肽的 MS/MS 谱进行匹配得到了验证。据我们所知，这是首次整合 OMIC 数据集鉴定发育中的鼠晶状体中新型肽的报告。
