蒺藜苜蓿根中微小RNA的稳定失活

Stable Inactivation of MicroRNAs in Medicago truncatula Roots.

作者信息

Proust Hélène, Bazin Jérémie, Sorin Céline, Hartmann Caroline, Crespi Martin, Lelandais-Brière Christine

机构信息

Institute of Plant Sciences Paris-Saclay, IPS2, Univ. Paris-Diderot, CNRS, INRA, Univ. Paris-Sud, Univ. Evry, Sorbonne Paris-Cité, University of Paris-Saclay, Orsay, France.

出版信息

Methods Mol Biol. 2018;1822:123-132. doi: 10.1007/978-1-4939-8633-0_9.

DOI:10.1007/978-1-4939-8633-0_9

PMID:30043301

Abstract

MicroRNAs are key regulators in the development processes or stress responses in plants. In the last decade, several conserved or non-conserved microRNAs have been identified in Medicago truncatula. Different strategies leading to the inactivation of microRNAs in plants have been described. Here, we propose a protocol for an effective inactivation of microRNAs using a STTM strategy in M. truncatula transgenic roots.

摘要

微小RNA是植物发育过程或应激反应中的关键调节因子。在过去十年中，已在蒺藜苜蓿中鉴定出几种保守或非保守的微小RNA。已经描述了导致植物中微小RNA失活的不同策略。在此，我们提出了一种使用STTM策略在蒺藜苜蓿转基因根中有效使微小RNA失活的方案。