Senf Deborah, Ruprecht Colin, Kishani Saina, Matic Aleksandar, Toriz Guillermo, Gatenholm Paul, Wågberg Lars, Pfrengle Fabian
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476, Potsdam, Germany.
Institute of Chemistry and Biochemistry, Freie Universität Berlin, Arnimallee 22, 14195, Berlin, Germany.
Angew Chem Int Ed Engl. 2018 Sep 10;57(37):11987-11992. doi: 10.1002/anie.201806871. Epub 2018 Aug 19.
The heterogeneous nature of non-cellulosic polysaccharides, such as arabinoxylan, makes it difficult to correlate molecular structure with macroscopic properties. To study the impact of specific structural features of the polysaccharides on crystallinity or affinity to other cell wall components, collections of polysaccharides with defined repeating units are required. Herein, a chemoenzymatic approach to artificial arabinoxylan polysaccharides with systematically altered branching patterns is described. The polysaccharides were obtained by glycosynthase-catalyzed polymerization of glycosyl fluorides derived from arabinoxylan oligosaccharides. X-ray diffraction and adsorption experiments on cellulosic surfaces revealed that the physicochemical properties of the synthetic polysaccharides strongly depend on the specific nature of their substitution patterns. The artificial polysaccharides allow structure-property relationship studies that are not accessible by other means.
非纤维素多糖（如阿拉伯木聚糖）的异质性使得难以将分子结构与宏观性质相关联。为了研究多糖特定结构特征对结晶度或与其他细胞壁成分亲和力的影响，需要具有确定重复单元的多糖集合。本文描述了一种化学酶法制备具有系统改变分支模式的人工阿拉伯木聚糖多糖的方法。这些多糖是通过糖基合酶催化源自阿拉伯木聚糖寡糖的糖基氟化物的聚合反应得到的。对纤维素表面的X射线衍射和吸附实验表明，合成多糖的物理化学性质强烈依赖于其取代模式的特定性质。这些人工多糖使得通过其他方法无法进行的结构-性质关系研究成为可能。
