Pepin C A, Wood H G, Robinson N A
Biochem Int. 1986 Jan;12(1):111-23.
A procedure for determining the size of inorganic polyphosphates of chain lengths up to about 750 is described. It involves reducing the size with polyphosphate glucokinase to a chain length that can be accurately determined by polyacrylamide gel electrophoresis. This measurement along with determination of the glucose-6-P formed and the total phosphate of the original polyphosphate permits calculation of the chain length. The accuracy of this method has been demonstrated by comparison with other reliable procedures. Thus far, it is the only method available for sizing long chain polyphosphates with nmol quantities.
本文描述了一种用于测定链长高达约750的无机多磷酸盐大小的方法。该方法包括用多磷酸盐葡萄糖激酶将其大小减小至可通过聚丙烯酰胺凝胶电泳准确测定的链长。这种测量以及对形成的6-磷酸葡萄糖和原始多磷酸盐总磷酸盐的测定允许计算链长。通过与其他可靠方法比较证明了该方法的准确性。到目前为止，它是唯一可用于对纳摩尔量的长链多磷酸盐进行大小测定的方法。
