Vassilyadi M, Archibald F
Can J Microbiol. 1985 Nov;31(11):994-9. doi: 10.1139/m85-188.
A number of facultatively anaerobic members of the genus Bacillus were screened for their catalase, diaminobenzidine peroxidase, and superoxide dismutase activities. A strain of Bacillus coagulans (7050) lacking peroxidatic activity and containing single catalatic and superoxide dismutase activities was selected. Responses of the superoxide dismutase activity and catalase level to the partial pressure of oxygen, and Fe and Mn levels, as well as to aerobic and fermentative metabolism, were determined. There appeared to be a relationship between high endogenous catalase levels and the high H2O2 evolution and KCN insensitivity of B. coagulans respiration. Bacillus coagulans 7050 was mutagenized with N-methyl-N'-nitro-N-nitrosoguanidine and screened for the expression of oxygen intolerance. All of the 38 stable oxygen sensitive mutants obtained had very low or completely absent catalatic activity and catalase protein. No mutant lacked superoxide dismutase, although five showed significantly lowered levels of the enzyme. Exogenous bovine liver catalase restored aerotolerance and reduced cell pleomorphism in the mutants.
对芽孢杆菌属的一些兼性厌氧菌进行了过氧化氢酶、二氨基联苯胺过氧化物酶和超氧化物歧化酶活性的筛选。选择了一株缺乏过氧化物酶活性、仅含有单一过氧化氢酶和超氧化物歧化酶活性的凝结芽孢杆菌（7050）。测定了超氧化物歧化酶活性和过氧化氢酶水平对氧分压、铁和锰水平以及对需氧和发酵代谢的反应。凝结芽孢杆菌的高内源性过氧化氢酶水平与高过氧化氢释放量以及对KCN不敏感的呼吸作用之间似乎存在关联。用N-甲基-N'-硝基-N-亚硝基胍对凝结芽孢杆菌7050进行诱变，并筛选对氧不耐受的表达。获得的38个稳定的氧敏感突变体均具有极低或完全没有过氧化氢酶活性和过氧化氢酶蛋白。没有突变体缺乏超氧化物歧化酶，尽管有5个突变体的该酶水平显著降低。外源性牛肝过氧化氢酶恢复了突变体的耐氧性并减少了细胞多形性。
