Damani-Yokota Payal, Gillespie Alexandria, Pasman Yfke, Merico Daniel, Connelley Timothy K, Kaushik Azad, Baldwin Cynthia L
Program in Molecular & Cellular Biology, University of Massachusetts, Amherst, MA, 01003, USA.
Department of Veterinary & Animal Sciences, University of Massachusetts, Amherst, MA, 01003, USA.
Dev Comp Immunol. 2018 Nov;88:190-199. doi: 10.1016/j.dci.2018.07.023. Epub 2018 Jul 23.
Here we evaluated neonatal transcription of α, β, γ and δ TCR and the γδ T cell co-receptor family WC1 in peripheral blood mononuclear cells. A previous report showed a rapid and global shift in transcription of immunoglobulin genes in neonatal calves during the first month after birth but this was not found here for the T cell genes. Transcription frequency of genes within TRAV subgroups correlated with the number of members, indicating a stochastic choice. In contrast, of the approximately 60 TRDV genes those in two of eleven TRDV1 clades and TRDVb3 were transcribed significantly more than the others while those in only one TRBV subgroup were. Transcription of genes in the TRGV5-containing cassette predominated among TRGV genes as a result of their exclusive usage by the WC1 γδ T cells with a preference for transcription of two of four TRGV genes in that cassette. Finally, we report no large differences in transcription frequencies among the 13 WC1 genes.
在此，我们评估了外周血单个核细胞中α、β、γ和δTCR以及γδT细胞共受体家族WC1的新生儿转录情况。先前的一份报告显示，新生犊牛出生后第一个月免疫球蛋白基因的转录会迅速发生全面变化，但此处未发现T细胞基因有这种情况。TRAV亚组内基因的转录频率与成员数量相关，表明是随机选择。相比之下，在大约60个TRDV基因中，11个TRDV1分支中的两个分支以及TRDVb3中的基因转录明显多于其他基因，而TRBV亚组中只有一个亚组的基因是这样。由于WC1γδT细胞专门使用含TRGV5的盒式结构中的基因，且偏好该盒式结构中四个TRGV基因中的两个基因进行转录，所以TRGV基因中该盒式结构中的基因转录占主导。最后，我们报告13个WC1基因的转录频率没有大的差异。
