Rajendra Memorial Research Institute of Medical Sciences, (Indian Council of Medical Research) Agam Kuan, Patna, 800 007, India.
Rajendra Memorial Research Institute of Medical Sciences, (Indian Council of Medical Research) Agam Kuan, Patna, 800 007, India.
Cell Immunol. 2018 Oct;332:51-57. doi: 10.1016/j.cellimm.2018.07.006. Epub 2018 Jul 18.
Visceral leishmaniasis (VL) is a disease caused by protozoan species of the genus Leishmania and is transmitted through bites from the Phlebotomus sand fly; it is associated with considerable morbidity and mortality in many parts of world, including India. Reports on the protective role played by saliva proteins of Lutozomyia longipalpis, Phlebotomus papatasi and Phlebotomus duboscqi. are available. However, no studies have explored the salivary proteins of P. argentipes, which is the known proven vector for the transmission of VL in the Indian sub-continent. Herein we revealed the presence of two proteins of 14.2 and one protein of 13.6 kDa in Indian strain P. argentipes which is absolute identical to previously reported protein of SP15 family (PagSP01, PagSP02 and PagSP07) of P. argentipes of NIH colony, USA. In an experimental study on P. argentipes from Bihar, India, we demonstrated that a strong humoral and cellular immune response was triggered to reduce the concomitant Leishmania load in groups of immunized mice. The immunized group produced a considerable amount of IgG antibodies, and their splenocytes generated TH1 cytokines (IL-12, IFN-γ) with the support of delayed-type hypersensitivity (DTH) reactivity in such mice at the challenged site. We summarize from our data that some identical proteins to previous from SP15 family protein of 14.2 and 13.6 kDa molecular size, derived from Indian P. argentipes and reported its first time, can also be significant in resolution of VL infection after modulation of host protective T cell response in VL.
内脏利什曼病（VL）是一种由利什曼原虫属的原生动物物种引起的疾病，通过沙蝇的叮咬传播；它在世界许多地区都与相当高的发病率和死亡率有关，包括印度。有报道称，长须白蛉、白蛉和白蛉的唾液蛋白在保护作用中发挥了作用。然而，尚无研究探讨 P. argentipes 的唾液蛋白，它是印度次大陆传播 VL 的已知有效传播媒介。在此，我们在印度 P. argentipes 中发现了两种 14.2 kDa 的蛋白和一种 13.6 kDa 的蛋白，与先前报道的美国 NIH 群体 P. argentipes 的 SP15 家族蛋白（PagSP01、PagSP02 和 PagSP07）绝对相同。在对来自印度比哈尔邦的 P. argentipes 的实验研究中，我们证明了强烈的体液和细胞免疫反应被触发，以减少免疫接种小鼠中伴随的利什曼原虫负荷。免疫组小鼠产生了大量的 IgG 抗体，其脾细胞在 challenged 部位产生了 TH1 细胞因子（IL-12、IFN-γ），并伴有迟发型超敏反应（DTH）反应。我们从数据中总结出，一些与先前报道的 SP15 家族蛋白相同的蛋白，大小为 14.2 和 13.6 kDa，来自印度的 P. argentipes，并首次报道了它，在调节 VL 宿主保护性 T 细胞反应后，也可能在 VL 感染的缓解中发挥重要作用。
