Popławska Magdalena, Błażewicz Agata
Counterfeit Medicinal Products and Designer Drugs Department, National Medicines Institute, Warsaw, Poland.
Drug Test Anal. 2019 Jan;11(1):162-167. doi: 10.1002/dta.2467. Epub 2018 Aug 14.
Growth hormone releasing peptides (GHRPs) are synthetic peptides with the ability to stimulate human growth hormone (hGH) secretion. Several GHRPs have been developed as drug candidates; however, only one of them, GHRP-2 (Pralmorelin), has received a clinical approval. Nevertheless, they are distributed on the black market and misused by cheating athletes, due to their performance-enhancing effects. Hence, GHRPs have been included in the World-Anti-Doping-Agency's Prohibited List as forbidden substances in sport. Predominantly, analytical methods for detection and unequivocal identification of doping substances are based on mass spectrometry. Therefore, in the present work, a qualitative analysis by liquid chromatography coupled to high-resolution tandem mass spectrometry with a quadrupole time-of-flight analyzer was performed to identify a new heptapeptide (MW = 874.02 Da) - a glycine analogue of GHRP-2. Structure determination using de novo sequencing is described here in detail. The results of this study may indicate a new approach to circumvent a detection of doping practices.
生长激素释放肽（GHRPs）是一类具有刺激人类生长激素（hGH）分泌能力的合成肽。几种GHRPs已被开发为候选药物；然而，其中只有一种，即GHRP - 2（普拉莫瑞林）获得了临床批准。尽管如此，由于它们具有增强体能的作用，在黑市上流通并被作弊运动员滥用。因此，GHRPs已被列入世界反兴奋剂机构的《禁用清单》，被列为体育赛事中的违禁物质。主要地，检测和明确识别兴奋剂物质的分析方法基于质谱法。因此，在本研究中，采用液相色谱与具有四极杆飞行时间分析仪的高分辨率串联质谱联用进行定性分析，以鉴定一种新的七肽（分子量 = 874.02 Da）——GHRP - 2的甘氨酸类似物。本文详细描述了使用从头测序进行结构测定的过程。本研究结果可能表明一种规避兴奋剂检测的新方法。
