Zheng Bin, Dong Jin-chao, Su Li-zhong, Meng Meng, Zhang Yue-jiao, Li Jian-feng
Guang Pu Xue Yu Guang Pu Fen Xi. 2016 Jun;36(6):1987-91.
In this work, we have mainly studied SERS spectra of fresh human urine by using Au nanoparticles excited by 785 and 1 030 nm lasers, respectively. And the UV/Vis adsorption experiment of the Au nanoparticles mixed with different ratio of urine has been performed, and the obvious shifting of corresponding absorption band is observed. The result showed that the Au nanoparticles which have been synthesized by classical Fren’s method can interact with urine, and the Au nanoparticles aggregations caused by the urine have strong SERS effect. Intense and repeatable spectra of the urine samples can be quickly obtained using Au colloids, which characterized by the scanning electron microscope (SEM) and the high-resolution transmission electron microscope (HRTEM) images, and it can be confirmed that the size of the Au nanoparticles is about 55 nm with a finite variation. When different spectra can be detected under different exciting lasers, the various biofluid to Au substrate ratios can generate different intense spectra. From the spectra of 785 nm laser, we can conclude that it has lower background and higher resolution with more detail information of this system contained human urine. For the 1 030 nm laser, a portable Raman instrument is helpful for on-site clinic treatment detection. It also gets well defined information and will be a good and convenient choice for urine analysis. It should note that this peak band located at 1 006 cm-1 may be the dominant nitrogen-containing component in urine. On the other hand, uric acid, urea, hypoxanthine as well as creatinine can be assigned; the other bands are still unknown, which might be attributed to biomarkers important for disease differentiation. Another result shows that different sample preparation can influence the SERS spectra with different ratio. We also have made a comparison of Raman spectra between 785 and 1 030 nm lasers to learn the difference between each other just like background and high-resolution. The current study indicates the SERS of urine might be a good choice and tool for urinalysis with potential diagnostic application, especially with the portable Raman instrument which would be an accurate and convenient approach for urine analysis. It is possible for SERS detection to be applied in not only the health diagnosis but also biological tissue in the future.
在本研究中，我们主要分别使用785纳米和1030纳米激光激发金纳米颗粒，研究了新鲜人尿液的表面增强拉曼光谱（SERS）。并且进行了金纳米颗粒与不同比例尿液混合后的紫外可见吸收实验，观察到相应吸收带的明显位移。结果表明，采用经典的弗伦德方法合成的金纳米颗粒能够与尿液相互作用，尿液引起的金纳米颗粒聚集具有很强的SERS效应。使用金胶体可以快速获得尿液样本的强而可重复的光谱，通过扫描电子显微镜（SEM）和高分辨率透射电子显微镜（HRTEM）图像对其进行表征，可以确认金纳米颗粒的尺寸约为55纳米，变化有限。当在不同的激发激光下可以检测到不同的光谱时，不同的生物流体与金基底比例会产生不同强度的光谱。从785纳米激光的光谱中，我们可以得出结论，它具有较低的背景和较高的分辨率，包含了该系统中人类尿液的更多详细信息。对于1030纳米激光，便携式拉曼仪器有助于现场临床治疗检测。它也能获得明确的信息，将是尿液分析的一个良好且方便的选择。需要注意的是，位于1006厘米-1处的这个峰带可能是尿液中主要的含氮成分。另一方面，可以确定尿酸、尿素、次黄嘌呤以及肌酐；其他谱带仍然未知，这可能归因于对疾病鉴别很重要的生物标志物。另一个结果表明，不同的样品制备方法会以不同比例影响SERS光谱。我们还比较了785纳米和1030纳米激光之间的拉曼光谱，以了解彼此之间在背景和高分辨率等方面的差异。当前的研究表明，尿液的SERS可能是尿液分析的一个良好选择和工具，具有潜在的诊断应用价值，特别是便携式拉曼仪器将是一种准确且方便的尿液分析方法。SERS检测不仅有可能应用于健康诊断，未来还有可能应用于生物组织。
