Taylor D S, Kern J A, Nowell P C
J Immunol. 1986 Mar 1;136(5):1620-4.
To investigate the ability of interleukin 2 (IL 2) alone to induce proliferation of resting human lymphocytes, we stimulated human peripheral blood mononuclear cells (PBMC) with an immunopurified preparation of IL 2 or with phytohemagglutinin (PHA). Proliferation and the percent of cells expressing IL 2 receptors were assessed over 6 days of culture. Regardless of the stimulus, the percent of cells bearing an IL 2 receptor paralleled the amount of proliferation, and proliferation was inhibited by an anti-IL 2 receptor monoclonal antibody (anti-Tac). When stimulated by IL 2 alone, less than 8% of PBMC expressed an IL 2 receptor after 24 hr of culture. Stimulation by IL 2 caused both proliferation and IL 2 receptor expression to increase over the entire culture period (routinely to 75,000 cpm and 50% respectively). When colchicine was added (to inhibit cell division), the percent of cells bearing an IL 2 receptor did not increase. IL 2 alone also induced proliferation of PBMC depleted of accessory cells, with the same kinetics but reduced peak response. Both accessory cells and supernatants that showed IL 1 but not IL 2 activity augmented this proliferation 50 to 100%. In contrast to the effect of IL 2, 25 to 50% of PBMC stimulated by PHA expressed an IL 2 receptor after 24 hr of culture. PHA-induced proliferation and IL 2 receptor expression peaked early in the culture period (routinely to 100,000 cpm and 50% respectively within 3 days), and colchicine did not inhibit the early induction of IL 2 receptors on PBMC. Our findings indicate that unlike PHA, IL 2 induces proliferation of PBMC (or PBMC depleted of accessory cells) by expanding the small percentage of cells in a resting population that already express IL 2 receptors. IL 2 does not appear to induce IL 2 receptors on cells previously lacking this molecule. We also find that IL 1 can enhance the response to IL 2 alone.
为了研究单独使用白细胞介素2（IL-2）诱导静息人淋巴细胞增殖的能力，我们用免疫纯化的IL-2制剂或植物血凝素（PHA）刺激人外周血单个核细胞（PBMC）。在6天的培养过程中评估细胞增殖及表达IL-2受体的细胞百分比。无论刺激物是什么，带有IL-2受体的细胞百分比与增殖量平行，并且增殖受到抗IL-2受体单克隆抗体（抗Tac）的抑制。当仅用IL-2刺激时，培养24小时后不到8%的PBMC表达IL-2受体。IL-2刺激导致在整个培养期内增殖和IL-2受体表达均增加（通常分别达到75,000 cpm和50%）。当加入秋水仙碱（以抑制细胞分裂）时，带有IL-2受体的细胞百分比没有增加。单独的IL-2也能诱导去除辅助细胞的PBMC增殖，增殖动力学相同但峰值反应降低。显示IL-1但不显示IL-2活性的辅助细胞和上清液均可使这种增殖增强50%至100%。与IL-2的作用相反，PHA刺激的PBMC在培养24小时后有25%至50%表达IL-2受体。PHA诱导的增殖和IL-2受体表达在培养期早期达到峰值（通常在3天内分别达到100,000 cpm和50%），并且秋水仙碱不抑制PBMC上IL-2受体的早期诱导。我们的研究结果表明，与PHA不同，IL-2通过使静息群体中已表达IL-2受体的小部分细胞扩增来诱导PBMC（或去除辅助细胞的PBMC）增殖。IL-2似乎不会在先前缺乏该分子的细胞上诱导IL-2受体。我们还发现IL-1可以增强对单独IL-2的反应。
