Morimoto T, Taki Y, Jikko A, Yokoo N, Koizumi K, Nishihira T, Nishikawa K, Moguchi M, Tanaka A, Tanaka J
J Lab Clin Med. 1986 Mar;107(3):194-8.
To clarify the functional adaptability of mitochondria in the regenerating liver, the concentrations of respiratory components, hepatic energy charge levels, cytochrome oxidase activity, and phosphorylative activity were studied in mitochondria obtained from regenerating liver of rats treated with chloramphenicol (CAP). In the hepatectomized groups with CAP treatment, a dose-dependent decrease occurred in the concentrations of cytochrome a(+ a3), cytochrome b, cytochrome c + c1, flavoprotein, and pyridine nucleotide. Cytochrome oxidase activity and phosphorylative activity per milligram of mitochondrial protein also decreased dose dependently in CAP-treated hepatectomized groups, with a significant increase in these values per unit of cytochrome a(+ a3). Hepatic energy charge levels significantly decreased in the hepatectomized groups. However, no significant differences were seen among the hepatectomized groups. However, no significant differences were seen among the hepatectomized groups with or without CAP treatment. Our results suggest that hepatic energy charge is maintained at the same relative level by a compensatory enhancement in phosphorylative capacity associated with cytochrome oxidase activity per unit of cytochrome a(+ a3), in spite of a remarkable decrease in the concentrations of respiratory components.
为阐明再生肝中线粒体的功能适应性，对氯霉素（CAP）处理的大鼠再生肝线粒体中的呼吸成分浓度、肝脏能量电荷水平、细胞色素氧化酶活性和磷酸化活性进行了研究。在CAP处理的肝切除组中，细胞色素a（+ a3）、细胞色素b、细胞色素c + c1、黄素蛋白和吡啶核苷酸的浓度呈剂量依赖性降低。每毫克线粒体蛋白的细胞色素氧化酶活性和磷酸化活性在CAP处理的肝切除组中也呈剂量依赖性降低，而每单位细胞色素a（+ a3）的这些值则显著增加。肝切除组的肝脏能量电荷水平显著降低。然而，肝切除组之间未见显著差异。不过，CAP处理组和未处理组的肝切除组之间未见显著差异。我们的结果表明，尽管呼吸成分的浓度显著降低，但通过与每单位细胞色素a（+ a3）的细胞色素氧化酶活性相关的磷酸化能力的代偿性增强，肝脏能量电荷维持在相同的相对水平。
