Unité de Biotechnologies et Bioprocédés, Université Libre de Bruxelles, Belgium.
Microbial Processes and Interactions, TERRA Teaching and Research Centre, University of Liège - Gembloux Agro-Bio Tech, Belgium.
Bioresour Technol. 2018 Jan;247:963-969. doi: 10.1016/j.biortech.2017.09.168. Epub 2017 Sep 27.
In this study, gene YALI0F01650g has been isolated and characterized. Several experimental evidences suggest that the identified gene, renamed EYD1, encodes an erythritol dehydrogenase. An efficient bioreactor process for the bioconversion of erythritol into erythrulose was also developed. Using constitutive expression of EYD1 in a Y. lipolytica mutant containing a disrupted EYK1 gene, which encodes erythrulose kinase, erythrulose could be synthesized from erythritol at a rate of 0.116g/g.h and with a bioconversion yield of 0.64g/g.
在这项研究中，基因 YALI0F01650g 已经被分离和鉴定。多项实验证据表明，该鉴定基因被重新命名为 EYD1，编码赤藓糖醇脱氢酶。我们还开发了一种从赤藓糖醇生物转化生产赤藓酮糖的高效生物反应器工艺。通过在含有一个破坏的 EYK1 基因的 Y. lipolytica 突变体中组成型表达 EYD1，该基因编码赤藓酮糖激酶，赤藓糖醇可以以 0.116g/g.h 的速度和 0.64g/g 的生物转化产率合成赤藓酮糖。
