Overbeek P A, Lai S P, Van Quill K R, Westphal H
Science. 1986 Mar 28;231(4745):1574-7. doi: 10.1126/science.3006249.
Transgenic mice were generated with pRSV-CAT, a chimeric gene construct containing the long terminal repeat of Rous sarcoma virus (RSV) linked to the bacterial gene encoding chloramphenicol acetyltransferase (CAT). CAT expression, detected in adult animals of five independent strains, was preferentially directed to organs rich in tendon, bone, and muscle. This pattern reflects the disease specificity of the intact virus and suggests that the tissue tropism of RSV is determined at least in part by the presence of endogenous tissue-specific factors that can promote expression of genetic information linked to the long terminal repeat. In two of the mouse strains, insertion of the pRSV-CAT DNA resulted in developmental abnormalities. One of these strains was characterized by a dominant trait of embryonic lethality, the other by a recessive trait of fused toes in all four feet.
利用pRSV-CAT构建了转基因小鼠，pRSV-CAT是一种嵌合基因构建体，包含劳斯肉瘤病毒（RSV）的长末端重复序列，该序列与编码氯霉素乙酰转移酶（CAT）的细菌基因相连。在五个独立品系的成年动物中检测到的CAT表达，优先定向到富含肌腱、骨骼和肌肉的器官。这种模式反映了完整病毒的疾病特异性，并表明RSV的组织嗜性至少部分由内源性组织特异性因子的存在所决定，这些因子可以促进与长末端重复序列相连的遗传信息的表达。在其中两个小鼠品系中，pRSV-CAT DNA的插入导致了发育异常。其中一个品系的特征是胚胎致死的显性性状，另一个品系的特征是所有四只脚的脚趾融合的隐性性状。
