Wainwright S D
Can J Biochem. 1977 Apr;55(4):415-23. doi: 10.1139/o77-058.
Marked differences were seen between the metabolism of L-[3-14C] tryptophan and of [2-14C]serotonin by the intact chick pineal gland in organ culture. The major metabolite of tryptophan recovered by our procedures was melatonin, which accounted for about half the radioactivity recovered as metabolic products. In contrast, the principal product of serotonin metabolism recovered was hydroxyindoleacetic acid, and the yield of products derived through monoamine oxidase (EC 1.4.3.4) activity vastly exceeded that of melatonin. Metabolism of tryptophan yielded a much larger proportion of methlated metabolites among the products recovered than did metabolism of serotonin. However, the yield of methoxyindoleacetic acid from serotonin was greater than that from tryptophan. Serotonin formed endogenously and serotonin supplied exogenously appear to enter two or more largely distinct metabolic pools.
在器官培养中，完整的鸡松果体对L-[3-¹⁴C]色氨酸和[2-¹⁴C]血清素的代谢存在显著差异。通过我们的实验步骤回收的色氨酸主要代谢产物是褪黑素，其占回收的代谢产物放射性的约一半。相比之下，回收的血清素代谢的主要产物是羟基吲哚乙酸，通过单胺氧化酶（EC 1.4.3.4）活性产生的产物产量远远超过褪黑素。色氨酸代谢产生的甲基化代谢产物在回收产物中的比例远高于血清素代谢。然而，血清素产生的甲氧基吲哚乙酸产量高于色氨酸。内源性形成的血清素和外源性提供的血清素似乎进入两个或更多基本不同的代谢池。
