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化学和天然抗褐变剂对姜(Z Roscoe)中多酚氧化酶的抑制作用

Inhibitory effect of chemical and natural anti-browning agents on polyphenol oxidase from ginger (Z Roscoe).

作者信息

Lim Win Yee, Wong Chen Wai

机构信息

Department of Biotechnology, Faculty of Applied Sciences, UCSI University, No. 1, Jalan Menara Gading, UCSI Heights, 56000 Kuala Lumpur, Malaysia.

出版信息

J Food Sci Technol. 2018 Aug;55(8):3001-3007. doi: 10.1007/s13197-018-3218-7. Epub 2018 May 19.

Abstract

Food browning is undesirable as it causes deterioration in food quality and appearance. This phenomenon was related to polyphenol oxidase (PPO), which catalyzes conversion of phenolic compounds into -quinones. The present work evaluated the use of chemical and natural anti-browning agents to prevent the browning of ginger PPO. Sodium metabisulfite (5 mM) is a better chemical inhibitor compared to l-cysteine and sodium chloride as 55.00% of ginger PPO inhibition was achieved. The percentage of inhibition increased as the concentration of anti-browning agents increases. The addition of heated onion, chili pepper and pineapple extracts exhibited a stronger inhibitory effect on ginger PPO than unheated extracts. Heated chili pepper extract was the best natural inhibitor found in this study and it inhibited the ginger PPO (47.97%) mixed-competitively. Natural anti-browning agents have potential to be used to control the browning of ginger as well as other vegetables and fruits.

摘要

食物褐变是不可取的,因为它会导致食物品质和外观变差。这种现象与多酚氧化酶(PPO)有关,该酶催化酚类化合物转化为醌类。本研究评估了使用化学和天然抗褐变剂来防止姜PPO褐变的效果。与L-半胱氨酸和氯化钠相比,焦亚硫酸钠(5 mM)是一种更好的化学抑制剂,因为它对姜PPO的抑制率达到了55.00%。随着抗褐变剂浓度的增加,抑制率也随之增加。添加加热过的洋葱、辣椒和菠萝提取物对姜PPO的抑制作用比未加热的提取物更强。加热过的辣椒提取物是本研究中发现的最佳天然抑制剂,它对姜PPO具有混合竞争性抑制作用(47.97%)。天然抗褐变剂有潜力用于控制姜以及其他蔬菜和水果的褐变。

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