Papadopoulos V, Drosdowsky M A, Carreau S
Andrologia. 1986 Jan-Feb;18(1):79-83. doi: 10.1111/j.1439-0272.1986.tb01742.x.
In Percoll purified adult rat Leydig cells, the estradiol secretion, in presence of exogenous testosterone (200 ng/ml) is stimulated 2-fold by either LH (100 ng/ml) or dbcAMP (1 mM). The addition of prolactin (1 microgram/ml) or dexamethasone (10(-7) M) to the Leydig cell incubation medium induces a 20% increase of the basal estradiol production, whereas, under LH or dbcAMP stimulations, 46 and 41% decreases are noted; moreover, a synergistic effect between prolactin and dexamethasone was observed in presence of dbcAMP leading to a 53% diminution of the estradiol synthesis. There results suggest that either hyperprolactinemia or high doses of glucocorticoids might inhibit the Leydig cell aromatase activity in presence of LH.
在经Percoll纯化的成年大鼠睾丸间质细胞中，在外源睾酮（200 ng/ml）存在的情况下，促黄体生成素（LH，100 ng/ml）或二丁酰环磷腺苷（dbcAMP，1 mM）均可使雌二醇分泌增加2倍。向睾丸间质细胞孵育培养基中添加催乳素（1微克/毫升）或地塞米松（10⁻⁷ M）可使基础雌二醇产量增加20%，而在LH或dbcAMP刺激下，雌二醇产量分别降低46%和41%；此外，在dbcAMP存在的情况下，观察到催乳素和地塞米松之间存在协同作用，导致雌二醇合成减少53%。这些结果表明，在LH存在的情况下，高催乳素血症或高剂量糖皮质激素可能会抑制睾丸间质细胞芳香化酶的活性。
