单体海鞘（柄海鞘）血淋巴中的半乳糖特异性凝集素。分子、结合及功能特性

Galactose-specific lectin in the hemolymph of solitary ascidian, Halocynthia roretzi. Molecular, binding and functional properties.

作者信息

Yokosawa H, Harada K, Igarashi K, Abe Y, Takahashi K, Ishii S

出版信息

Biochim Biophys Acta. 1986 Mar 28;870(2):242-7. doi: 10.1016/0167-4838(86)90228-1.

Abstract

Galactose-specific lectin isolated from the hemolymph of solitary ascidian, Halocynthia roretzi, has been further characterized. The hemagglutinating activity of the lectin is Ca2+-dependent. The lectin has a large molecular form as revealed by gel-permeation chromatography, sedimentation equilibrium and velocity measurement, and electron microscopic observation. The lectin is adsorbed to columns of blue-Sepharose and phenyl-Sepharose, and eluted with ethylene glycol, not with lactose or high concentration of NaCl. The lectin shows a stimulatory effect on the superoxide anion production by guinea-pig polymorphonuclear leukocytes, and the effect is inhibited, among various sugars, most strongly by melibiose.

摘要

从独居海鞘（柄海鞘）的血淋巴中分离出的半乳糖特异性凝集素已得到进一步鉴定。该凝集素的血凝活性依赖于Ca2+。通过凝胶渗透色谱、沉降平衡和速度测量以及电子显微镜观察发现，该凝集素具有大分子形式。该凝集素可吸附到蓝色琼脂糖柱和苯基琼脂糖柱上，并用乙二醇洗脱，而不是用乳糖或高浓度NaCl洗脱。该凝集素对豚鼠多形核白细胞产生超氧阴离子具有刺激作用，在各种糖类中，蜜二糖对该作用的抑制作用最强。