Porzsolt F, Janik R, Heil G, Brudler O, Raghavachar A, Scholz S, Papendick U, Heimpel H
Blut. 1986 Mar;52(3):185-90. doi: 10.1007/BF00320535.
Since the application of low doses of IFN-alpha is necessary to maintain remissions in Hairy Cell Leukemia (HCL) it is of interest whether peripheral blood mononuclear cells (MNC) of HCL patients can be induced in vitro to produce IFN-alpha. 9 patients suffering from advanced HCL were included in the study. The diagnoses were confirmed by characteristic findings in peripheral blood and bone marrow biopsies. For IFN treatment we initially used natural IFN-alpha (Bioferon) and switched later to recombinant IFN-alpha2 (Boehringer). MNC of 5 patients before IFN therapy and of 6 patients during IFN therapy (2-47 weeks) were induced by phytohemagglutinin (PHA), Corynebacterium parvum (C.p.), and sendai virus (SV). PHA is known to induce IFN-gamma. Both, C.p. and SV induced IFN-alpha but no IFN-gamma in MNC of healthy controls and of IFN treated breast cancer patients. In HCL patients normal antiviral activities could be induced by PHA. Zero or only low antiviral activities could be induced in MNC from 9 patients tested on 22 occasions. It is concluded that MNC from patients with advanced HCL can be induced to produce IFN-gamma but no IFN-alpha. Since IFN-alpha but not IFN-gamma is produced by monocytes it is likely that reduced numbers of monocytes which were found in our HCL patients before and during IFN treatment account for the described deficiency of IFN-alpha production.
由于低剂量干扰素-α的应用对于维持毛细胞白血病（HCL）的缓解是必要的，因此研究HCL患者的外周血单个核细胞（MNC）是否能在体外被诱导产生干扰素-α就很有意义。9例晚期HCL患者被纳入该研究。外周血和骨髓活检的特征性发现证实了诊断。对于干扰素治疗，我们最初使用天然干扰素-α（Bioferon），后来改用重组干扰素-α2（勃林格）。5例患者在干扰素治疗前以及6例患者在干扰素治疗期间（2 - 47周）的MNC，分别用植物血凝素（PHA）、短小棒状杆菌（C.p.）和仙台病毒（SV）进行诱导。已知PHA可诱导干扰素-γ。C.p.和SV在健康对照者以及接受干扰素治疗的乳腺癌患者的MNC中均可诱导产生干扰素-α，但不诱导产生干扰素-γ。在HCL患者中，PHA可诱导正常的抗病毒活性。在22次检测中，对9例患者的MNC进行检测时，均未诱导出或仅诱导出低抗病毒活性。得出的结论是，晚期HCL患者的MNC可被诱导产生干扰素-γ，但不能产生干扰素-α。由于单核细胞可产生干扰素-α而非干扰素-γ，在我们的HCL患者中，干扰素治疗前和治疗期间发现的单核细胞数量减少，可能是所描述的干扰素-α产生不足的原因。
