Guo Rui, Zhang Wenya, Yang Ying, Ding Jian, Ai Shiwei, Yang Wenzhi, Bai Xiaojuan, Zhang Yingmei
Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University, Tianshui South Road 222, Lanzhou, 730000, People's Republic of China.
Bull Environ Contam Toxicol. 2018 Sep;101(3):305-313. doi: 10.1007/s00128-018-2408-3. Epub 2018 Aug 1.
The present study aimed to investigate the effects of environmental heavy metals on sperm morphology and motility of Bufo raddei. We collected B. raddei from Baiyin (BY) and Liujiaxia (LJX); BY is an area heavily polluted by heavy metals, while LJX is a relatively unpolluted area. Significant accumulations of copper, zinc, lead and cadmium were observed in B. raddei from BY, together with relatively smaller sperm size and higher sperm abnormality rate. Unexpectedly, sperm velocity, percentages of sperm motility and viability of B. raddei from BY were significantly higher than those from LJX. Similarly, when sperm was suspended in heavy metal solutions, sperm velocity, percentages of sperm motility and viability significantly increased compared with those suspended in distilled water. These results indicate that environmental heavy metal stress may decrease sperm size, meanwhile enhance sperm motility through increasing the ratio of head length to total sperm length of B. raddei.
本研究旨在探讨环境重金属对花背蟾蜍精子形态和活力的影响。我们从白银（BY）和刘家峡（LJX）采集了花背蟾蜍；BY是一个受重金属严重污染的地区，而LJX是一个相对未受污染的地区。在来自BY的花背蟾蜍中观察到铜、锌、铅和镉的显著积累，同时精子尺寸相对较小且精子异常率较高。出乎意料的是，来自BY的花背蟾蜍的精子速度、精子活力百分比和存活率显著高于来自LJX的花背蟾蜍。同样，当精子悬浮在重金属溶液中时，与悬浮在蒸馏水中相比，精子速度、精子活力百分比和存活率显著增加。这些结果表明，环境重金属胁迫可能会减小精子尺寸，同时通过增加花背蟾蜍精子头部长度与精子总长度的比例来提高精子活力。
