Department of Obstetrics, Dezhou People's Hospital, Dezhou, China.
Eur Rev Med Pharmacol Sci. 2018 Aug;22(15):4820-4827. doi: 10.26355/eurrev_201808_15617.
The role of long non-coding RNA (lncRNA) H19 in endometrial carcinoma was studied, and its mechanism was also explored.
Quantitative Reverse Transcriptase-Polymerase Chain Reaction (qRT-PCR) was used to detect the expression of lncRNA H19, miR-612, and HOXA10 in endometrial carcinoma, and the relationship between lncRNA H19 and survival time was analyzed. The high expression or knockdown of lncRNA H19 in endometrial cancer cells was completed by cell transfection experiments. Cell counting kit-8 (CCK-8) assay was used to detect changes in the viability of endometrial cancer cells. Dual luciferase reporter assay was performed to verify that miR-612 could bind to lncRNA H19 or HOXA10. QRT-PCR and Western Blot assays were used to detect changes in the expression of HOXA10 in endometrial cancer cells before and after overexpression or knockdown of lncRNA H19.
The expression of lncRNA H19 and HOXA10 was high in endometrial carcinoma and miR-612 was lowly expressed. The survival curve suggested that lncRNA H19 was negatively correlated with patient survival. The mRNA expression of lncRNA H19 in endometrial cancer cells, including HEC1-A, HEC1-B, AN3CA, and Ishikawa, was detected by qRT-PCR. It was found that the expression of lncRNA H19 was highest in AN3CA and lowest in Ishikawa. The cell transfection experiments allowed Ishikawa cells to overexpress lncRNA H19 and AN3CA cells to reduce lncRNA H19 expression. After overexpression of lncRNA H19, the viability of Ishikawa cells as well as the mRNA and protein levels of HOXA10 increased. However, after knocking down lncRNA H19, the viability of AN3CA cells along with the mRNA and protein levels of HOXA10 decreased. The dual luciferase reporter assay results suggested that miR-612 could bind to lncRNA H19 and HOXA10.
The high expression of lncRNA H19 in endometrial carcinoma may regulate the expression level of its target gene HOXA10 by targeting miR-612, thus promoting cell proliferation to play a role in the development of endometrial carcinoma.
研究长链非编码 RNA（lncRNA）H19 在子宫内膜癌中的作用，并探讨其机制。
采用定量逆转录-聚合酶链反应（qRT-PCR）检测子宫内膜癌中 lncRNA H19、miR-612 和 HOXA10 的表达，分析 lncRNA H19 与生存时间的关系。通过细胞转染实验完成子宫内膜癌细胞中 lncRNA H19 的高表达或敲低。细胞计数试剂盒-8（CCK-8）检测子宫内膜癌细胞活力的变化。双荧光素酶报告基因实验验证 miR-612 可与 lncRNA H19 或 HOXA10 结合。qRT-PCR 和 Western Blot 检测 lncRNA H19 过表达或敲低前后子宫内膜癌细胞中 HOXA10 的表达变化。
子宫内膜癌中 lncRNA H19 和 HOXA10 的表达水平较高，miR-612 的表达水平较低。生存曲线提示 lncRNA H19 与患者生存呈负相关。qRT-PCR 检测子宫内膜癌细胞 HEC1-A、HEC1-B、AN3CA 和 Ishikawa 中 lncRNA H19 的 mRNA 表达，发现 lncRNA H19 在 AN3CA 中的表达最高，在 Ishikawa 中的表达最低。细胞转染实验使 Ishikawa 细胞过表达 lncRNA H19，使 AN3CA 细胞降低 lncRNA H19 的表达。过表达 lncRNA H19 后，Ishikawa 细胞的活力以及 HOXA10 的 mRNA 和蛋白水平均升高。然而，敲低 lncRNA H19 后，AN3CA 细胞的活力以及 HOXA10 的 mRNA 和蛋白水平均降低。双荧光素酶报告基因实验结果表明，miR-612 可以与 lncRNA H19 和 HOXA10 结合。
子宫内膜癌中 lncRNA H19 的高表达可能通过靶向 miR-612 调节其靶基因 HOXA10 的表达水平，从而促进细胞增殖，在子宫内膜癌的发生发展中发挥作用。
