Suganuma N, Seo H, Narita O, Tomoda Y, Matsui N
Nihon Sanka Fujinka Gakkai Zasshi. 1986 Feb;38(2):253-60.
A human prolactin (PRL) cDNA clone was digested with restriction enzyme Pst I and the resultant fragments were cloned into bacteriophage M13mp9. Single stranded recombinant DNAs having a coding strand of the PRL cDNA were selected by hybridization with 125I-labeled PRL mRNA obtained from human prolactinoma tissue. One of the single stranded recombinant DNAs was purified by agarose gel electrophoresis and labeled with 125I to a specific activity of 1.4 X 10(8) cpm per microgram of DNA. The probe could be successfully used in RNA dot hybridization. Analysis of poly (A) RNAs from prolactinoma, liver and placental tissues revealed that this probe was specific to PRL mRNA sequence. Hybridization of poly (A) RNA from decidual tissue to this probe revealed the presence of PRL mRNA sequence. However, PRL mRNA in decidua was at least 20,000 times less than that in pituitary prolactinoma.
用人催乳素（PRL）cDNA克隆用限制性内切酶Pst I消化，所得片段克隆到噬菌体M13mp9中。通过与从人催乳素瘤组织获得的125I标记的PRL mRNA杂交，选择具有PRL cDNA编码链的单链重组DNA。其中一个单链重组DNA通过琼脂糖凝胶电泳纯化，并用125I标记，比活性为每微克DNA 1.4×10（8）cpm。该探针可成功用于RNA斑点杂交。对催乳素瘤、肝脏和胎盘组织的多聚（A）RNA分析表明，该探针特异于PRL mRNA序列。蜕膜组织的多聚（A）RNA与该探针杂交显示存在PRL mRNA序列。然而，蜕膜中的PRL mRNA比垂体催乳素瘤中的至少少20000倍。
