Malinow R, Miller J P
Nature. 1986;320(6062):529-30. doi: 10.1038/320529a0.
Activity-induced changes in the efficacy of synaptic transmission between neurones are central to several prominent theories of learning. In both in vivo and in vitro preparations of the hippocampus, a conditioning high-frequency stimulus delivered to afferent fibres results in a long-term potentiation of synaptic transmission at those inputs. Evidence has been provided supporting both presynaptic and postsynaptic sites as loci where critical events occur in the development of potentiation. In this study we report that long-term potentiation is reversibly blocked by intracellular injection of hyperpolarizing current in the postsynaptic cell during the conditioning high-frequency stimulus, suggesting the involvement of a voltage-dependent postsynaptic mechanism.
神经元之间突触传递效能的活动诱导变化是几种重要学习理论的核心。在海马体的体内和体外制备中，施加于传入纤维的条件高频刺激会导致这些输入处突触传递的长期增强。已有证据支持突触前和突触后位点均为增强发展过程中关键事件发生的位点。在本研究中，我们报告在条件高频刺激期间，通过向突触后细胞内注射超极化电流可使长期增强作用被可逆性阻断，这表明存在一种电压依赖性突触后机制。
