Zhang Hui, Yang Yuhui, Yan Biao, Tang Xue, Cheng Xiangrong, Le Guowei, Shi Yonghui
State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi 214122, China.
Wei Sheng Yan Jiu. 2018 May;47(3):345-351.
To study the effects of oxidized tyrosine products and dityrosine on the myocardial injury and inflammatory response in 10-week-gavaged mice.
A total of 30 female Kunming mice were assigned to three groups: gavagedwith saline( Con), oxidized tyrosine products( O-Tyr) and dityrosine( Dityr) for 320μg/kg BW for 10 weeks. Levels of oxidized protein products( DT, AOPPs, 3-NT) and lipid peroxidation products( MDA), oxidative stress( T-AOC and GSH/GSSG), markers of myocardium injury( CK, CK-MB, cTnI and Ca~(2+)-ATPase), markers of inflammatory factor( CRP and TNF-α) were investigated and the genes related to inflammatory response were detected by Real-time quantitative( PCR).
10 weeks of gavage experiments enhanced the levels of dityrosine( DT), advanced oxidation protein products( AOPPs), 3-nitrotyrosine( 3-NT), and malondialdehyde( MDA), and decreased total antioxidant capacity( T-AOC) and the ratio of reduced glutathione to oxidized glutathione( GSH/GSSG) in mice plasma and myocardium. Besides, O-Tyr and Dityr increased the levels of creatine kinase( CK), creatine kinase isoenzymes( CK-MB), cardiac troponin I( cTnI) in plasma anddecreased the activities of Ca~(2+)-ATPase in myocardium. O-Tyr and Dityr increased the levels of C-reactive protein( CRP) and tumour necrosis factor α( TNF-α) in plasma. The gene expression of inflammatory response were up-regulated.
O-Tyr and Dityr increase the accumulation of myocardial protein oxidation and lipid peroxidation products and induce oxidative damage to myocardium. O-Tyr and Dityr may cause myocardial tissue injury and inflammatory response. Dityrosine, as the main component of tyrosine oxidative products, may play a major role in the process of oxidized tyrosine products causing myocardial injury in mice.
研究氧化酪氨酸产物和二酪氨酸对灌胃10周小鼠心肌损伤及炎症反应的影响。
将30只雌性昆明小鼠分为三组：分别用生理盐水（Con）、氧化酪氨酸产物（O-Tyr）和二酪氨酸（Dityr）按320μg/kg体重灌胃10周。检测氧化蛋白产物（DT、AOPPs、3-NT）和脂质过氧化产物（MDA）水平、氧化应激（T-AOC和GSH/GSSG）、心肌损伤标志物（CK、CK-MB、cTnI和Ca²⁺-ATPase）、炎症因子标志物（CRP和TNF-α），并通过实时定量聚合酶链反应（PCR）检测炎症反应相关基因。
10周灌胃实验使小鼠血浆和心肌中二酪氨酸（DT）、晚期氧化蛋白产物（AOPPs）、3-硝基酪氨酸（3-NT）及丙二醛（MDA）水平升高，总抗氧化能力（T-AOC）及还原型谷胱甘肽与氧化型谷胱甘肽比值（GSH/GSSG）降低。此外，O-Tyr和Dityr使血浆中肌酸激酶（CK）、肌酸激酶同工酶（CK-MB）、心肌肌钙蛋白I（cTnI）水平升高，心肌中Ca²⁺-ATPase活性降低。O-Tyr和Dityr使血浆中C反应蛋白（CRP）和肿瘤坏死因子α（TNF-α）水平升高，炎症反应相关基因表达上调。
O-Tyr和Dityr增加心肌蛋白氧化和脂质过氧化产物的蓄积，诱导心肌氧化损伤。O-Tyr和Dityr可能导致心肌组织损伤和炎症反应。二酪氨酸作为酪氨酸氧化产物的主要成分，可能在氧化酪氨酸产物致小鼠心肌损伤过程中起主要作用。
