Ibaraki Kyoko, Mizuno Makoto, Aoki Hitomi, Niwa Ayumi, Iwamoto Ikuko, Hara Akira, Tabata Hidenori, Ito Hidenori, Nagata Koh-Ichi
Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, Aichi 480-0392, Japan.
Department of Tissue and Organ Development, Gifu University Graduate School of Medicine, Gifu, Japan.
Acta Histochem Cytochem. 2018 Jun 26;51(3):119-128. doi: 10.1267/ahc.18009. Epub 2018 Jun 20.
ARHGEF9, also known as Collybistin, a guanine nucleotide exchange factor for Rho family GTPases, is thought to play an essential role in the mammalian brain. In this study, we prepared a specific polyclonal antibody against ARHGEF9, anti-ARHGEF9, and carried out expression analyses with mouse tissues especially brain. Western blotting analyses demonstrated tissue-dependent expression profiles of ARHGEF9 in the young adult mouse, and strongly suggested a role during brain development. Immunohistochemical analyses revealed developmental stage-dependent expression profiles of ARHGEF9 in cerebral cortex, hippocampus and cerebellum. ARHGEF9 exhibited partial localization at dendritic spines in cultured hippocampal neurons. From the obtained results, anti-ARHGEF9 was found to be a useful tool for biochemical and cell biological analyses of ARHGEF9.
ARHGEF9，也被称为Collybistin，是一种Rho家族GTP酶的鸟嘌呤核苷酸交换因子，被认为在哺乳动物大脑中发挥着至关重要的作用。在本研究中，我们制备了一种针对ARHGEF9的特异性多克隆抗体，即抗ARHGEF9，并对小鼠组织尤其是大脑进行了表达分析。蛋白质印迹分析表明，在成年小鼠中，ARHGEF9的表达具有组织依赖性，这强烈提示了其在大脑发育过程中的作用。免疫组织化学分析揭示了ARHGEF9在大脑皮层、海马体和小脑中的发育阶段依赖性表达模式。在培养的海马神经元中，ARHGEF9在树突棘处有部分定位。根据所得结果，发现抗ARHGEF9是用于ARHGEF9生化和细胞生物学分析的有用工具。
