Chao M V, Bothwell M A, Ross A H, Koprowski H, Lanahan A A, Buck C R, Sehgal A
Science. 1986 Apr 25;232(4749):518-21. doi: 10.1126/science.3008331.
Nerve growth factor (NGF) and its receptor are important in the development of cells derived from the neural crest. Mouse L cell transformants have been generated that stably express the human NGF receptor gene transfer with total human DNA. Affinity cross-linking, metabolic labeling and immunoprecipitation, and equilibrium binding with 125I-labeled NGF revealed that this NGF receptor had the same size and binding characteristics as the receptor from human melanoma cells and rat PC12 cells. The sequences encoding the NGF receptor were molecularly cloned using the human Alu repetitive sequence as a probe. A cosmid clone that contained the human NGF receptor gene allowed efficient transfection and expression of the receptor.
神经生长因子（NGF）及其受体在源自神经嵴的细胞发育中起重要作用。已产生稳定表达通过总人DNA进行基因转移的人NGF受体的小鼠L细胞转化体。亲和交联、代谢标记和免疫沉淀以及与125I标记的NGF的平衡结合表明，该NGF受体与来自人黑色素瘤细胞和大鼠PC12细胞的受体具有相同的大小和结合特性。使用人Alu重复序列作为探针分子克隆了编码NGF受体的序列。包含人NGF受体基因的黏粒克隆允许受体的有效转染和表达。
