Armin Shahnaz, Fallah Fatemeh, Azimi Leila, Samadi Kafil Hossein, Ghazvini Kiarash, Hasanzadeh Sepide, Karimi Abdollah
Pediatric Infections Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Drug Applied Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Cell Mol Biol (Noisy-le-grand). 2018 Jul 30;64(10):125-129.
NDM-1 producing gram-negative bacteria can be resistant to every beta-lactam antibiotic, including carbapenem which is one of the last-lines of antibiotic therapy against multi-drug resistant bacteria. This study aimed to detect the metallo-beta-lactamase in the isolated gram-negative bacteria of the Iranian clinical specimens collected from two major cities in Iran. In this cross sectional study 171 Acinetobacter baumannii, 120 Enterobacter spp. and 145 Klebsiella pneumoniae isolated from clinical specimens of two training hospitals in Tabriz and Mashhad were evaluated. Carbapenem resistant screening was performed according to CLSI guide line. The antibiotic susceptibility testing was prepared for carbapenem resistant strains. Then, the metallo-beta- lactamase genes detection was also carried out by PCR assay and confirmed by sequencing. Sixty-eight, 12 and 22 carbapenem resistant Acinetobacter baumannii, Klebsiella pneumoniae and Enterobacter spp. were respectively confirmed, respectively. blaVIM in 9% and blaNDM-1 in 4% of isolated A. baumannii were observed. blaNDM-1 was also detected in 18% and 25% of K. pneumoniae and Enterobacter spp. isolates, respectively. This is the first report of NDM-1 producer A. baumannii and Enterobacter pp. in Iran. NDM-1 producing gram-negative bacteria can be resistant to all beta-lactam antibiotics and cause complicated challenges in health care systems.
产NDM-1的革兰氏阴性菌可对每一种β-内酰胺类抗生素耐药，包括碳青霉烯类，而碳青霉烯类是针对多重耐药菌的最后一道抗生素防线之一。本研究旨在检测从伊朗两个主要城市收集的临床标本中分离出的革兰氏阴性菌中的金属β-内酰胺酶。在这项横断面研究中，对从大不里士和马什哈德的两家培训医院的临床标本中分离出的171株鲍曼不动杆菌、120株肠杆菌属细菌和145株肺炎克雷伯菌进行了评估。根据CLSI指南进行碳青霉烯类耐药性筛查。对碳青霉烯类耐药菌株进行抗生素敏感性测试。然后，通过PCR检测金属β-内酰胺酶基因，并通过测序进行确认。分别确认了68株、12株和22株碳青霉烯类耐药鲍曼不动杆菌、肺炎克雷伯菌和肠杆菌属细菌。在分离出的鲍曼不动杆菌中，观察到9%的blaVIM和4%的blaNDM-1。在肺炎克雷伯菌和肠杆菌属分离株中，分别有18%和25%检测到blaNDM-1。这是伊朗首次报道产NDM-1的鲍曼不动杆菌和肠杆菌属。产NDM-1的革兰氏阴性菌可对所有β-内酰胺类抗生素耐药，并给医疗保健系统带来复杂挑战。
