用于筛查肝素诱导的血小板减少症的PIFA PLUSS P4检测法
PIFA PLUSS P4 Assay for Screening of Heparin-Induced Thrombocytopenia.
作者信息
Compton Frances B, Alrabeh Reem, Nguyen Lisa Q, Nedelcu Elena, Wahed Amer, Nguyen Nghia D
机构信息
Department of Pathology & Laboratory Medicine, University of Texas Health Science Center at Houston.
Department of Pathology and Genomic Medicine, Houston Methodist Hospital.
出版信息
Lab Med. 2019 Jan 1;50(1):73-77. doi: 10.1093/labmed/lmy048.
BACKGROUND
The PIFA PLUSS PF4 Rapid Assay (PIFA) is a rapid screening test used for the diagnosis of heparin-induced thrombocytopenia (HIT).
OBJECTIVE
To determine the usefulness of this assay as a screening method in our institution.
METHODS
A total of 159 specimens from patients with suspected HIT were included in our study. We simultaneously performed PIFA assay and confirmatory polyspecific enzyme-linked immunosorbent assay (ELISA). We subjected most of the specimens with false-negative results to serotonin release assay (SRA).
RESULTS
The initial sensitivity and specificity of the PIFA assay were calculated as 27.3% and 71.5%, respectively. A total of 12 of 16 false-negative results were further tested using the SRA method. The revised sensitivity and specificity were 50.0% and 73.5%, respectively.
CONCLUSIONS
Despite its appealing feature of yielding rapid results, the PIFA assay is inadequate as a sole screening test for HIT because of its high probability of missing many true cases of HIT.
背景
PIFA PLUS PF4快速检测法（PIFA）是一种用于诊断肝素诱导的血小板减少症（HIT）的快速筛查试验。
目的
确定该检测法在本机构作为筛查方法的实用性。
方法
本研究纳入了159例疑似HIT患者的标本。我们同时进行了PIFA检测和确证性多特异性酶联免疫吸附试验（ELISA）。我们对大多数假阴性结果的标本进行了血清素释放试验（SRA）。
结果
PIFA检测法的初始敏感性和特异性分别计算为27.3%和71.5%。使用SRA方法对16例假阴性结果中的12例进行了进一步检测。修订后的敏感性和特异性分别为50.0%和73.5%。
结论
尽管PIFA检测法具有快速出结果这一吸引人的特点，但由于其漏诊许多真正HIT病例的可能性很高，因此作为HIT的唯一筛查试验并不充分。
Zotero 插件