Virology Research and Diagnostic Laboratory, Department of Microbiology, King George's Medical University, Lucknow, India.
J Med Virol. 2018 Dec;90(12):1875-1881. doi: 10.1002/jmv.25277. Epub 2018 Sep 3.
The worldwide prevalence of hepatitis C virus infection (HCV) is nearly 150 to 170 million cases. The prevalence of HCV infection in India is estimated to be around 1%. In India HCV genotype (GT)3 is the predominant GT followed by GT1. Our study aims to establish the prevalent GTs/subtypes of HCV circulating in Uttar Pradesh, North India, as reported from a tertiary care hospital.
The study was a retrospective observational analysis of consecutive 404 HCV RNA positive cases referred to our hospital from September 2014 to April 2017, and was approved by an institutional ethics committee. Written informed consent was taken from each participant. Clinical and demographic details of these patients were recorded using predesigned questionnaires. All the laboratory testing was carried out on a stored serum sample of enrolled cases. Genotyping of all 404 strains was done by Sanger's sequencing of the core region. The phylogenetic analysis of 179 HCV strains with a high-quality sequencing data was performed.
The distributions of prevalent GTs/subtypes as noted in the current study were ( n [%]): GT1a, 101 (25%); GT1b, 12 (2.9%); GT1c, 1 (0.25%); GT3a, 275 (68.07%); GT3b, 9 (2.2%); GT3g, 2 (0.49%); GT3i, 3 (0.74%); and GT4a, 1 (0.24%). HCV GTs GT2, GT5, and GT6 were not detected from our region. Sequence analysis showed high genotypic variability in HCV GT3. Phylogenetic analysis showed that HCV GT3 and GT1 circulating in our region were related to Indian strains reported earlier.
HCV GTs 3a and 1a are the commonest circulating GTs in Uttar Pradesh, India.
全球丙型肝炎病毒（HCV）感染率接近 1.5 亿至 1.7 亿例。印度 HCV 感染率估计在 1%左右。在印度，HCV 基因型（GT）3 是主要 GT，其次是 GT1。本研究旨在确定印度北方邦（Uttar Pradesh）流行的 HCV 循环 GT/亚型，这是从一家三级护理医院报告的。
本研究为回顾性观察性分析，纳入了 2014 年 9 月至 2017 年 4 月期间我院连续转诊的 404 例 HCV RNA 阳性病例，并获得了机构伦理委员会的批准。每位参与者均签署了书面知情同意书。使用预先设计的问卷记录这些患者的临床和人口统计学细节。对所有 404 株进行基因分型，采用 Sanger 测序对核心区进行测序。对 179 株具有高质量测序数据的 HCV 株进行了系统发育分析。
本研究中发现的流行 GT/亚型分布为（n[％]）：GT1a，101（25％）；GT1b，12（2.9％）；GT1c，1（0.25％）；GT3a，275（68.07％）；GT3b，9（2.2％）；GT3g，2（0.49％）；GT3i，3（0.74％）；和 GT4a，1（0.24％）。本地区未检测到 HCV GT2、GT5 和 GT6。序列分析显示 HCV GT3 具有高度的基因型变异性。系统发育分析显示，本地区流行的 HCV GT3 和 GT1 与之前报道的印度株有关。
HCV GT3a 和 GT1a 是印度北方邦最常见的流行 GT。
