Valencia R, Espinosa R, Borovoy N, Pérez S, Ceja I, Saadia M
J Clin Pediatr Dent. 2018;42(6):434-441. doi: 10.17796/1053-4625-42.6.5. Epub 2018 Aug 7.
The goal of this in vitro study was to identify whether occlusal enamel deproteinization is effective in the removal of organic material in order to obtain quality etching patterns using phosphoric acid (HPO) and sodium hypochlorite (NaOCl) compared to phosphoric acid alone.
Nine extracted third permanent molars were polished with pumice and water. Every pit and fissure was evaluated as a unit, resulting in 40 individual units and then these were divided into five treatment groups. The occlusal enamel surface of each group was subjected to the following treatments: Group 1 (C) Control: No treatment; Group 2 (P): Polish and rinse; Group 3 (PD): Polish, rinse, and sodium hypochlorite (NaOCl) 5.25% for 60 seconds; Group 4 (PA): Polish, rinse, and acid etching with HPO 37% for 15 seconds; and Group 5 (PDA): Polish, rinse, sodium hypochlorite (NaOCl) 5.25% for 60 seconds, and acid etching with HPO 37% for 15 seconds. Results showed no significant statistical difference in the organic material present between groups 1 (C) (30.18%) and 2 (P) (36.61%), but there was a statistical difference (p <0.002) between Groups 1 and 2, and Group 3 (PD) (16.50%). In the acid etching group, the undesirable Type-III pattern (discussed later) was found in Group 4 (PA) (33.54%), while this was only 7.70% in Group 5, nearly five times more than Group 4, with a significant statistical difference (0.05). When differences were sought for Types I and II etch patterns (discussed later) for Groups 4 and 5, Group 4 (PA) obtained 26.29% (Type I) and 1.75% (Type II) etch patterns, compared to Group 5 (PDA) with 33.4% (Type I) and 38.97% (Type II) etch patterns.
The enamel deproteinization technique is an effective way to remove organic material on the occlusal surfaces of teeth, obtaining after phosphoric acid application, up to 72.38% of Types I and II etch patterns. Etching Types I or II can also be determined by the removal of organic material in between enamel crystals.
本体外研究的目的是确定咬合面釉质脱蛋白在去除有机物质方面是否有效，以便与单独使用磷酸相比，使用磷酸（HPO）和次氯酸钠（NaOCl）获得高质量的蚀刻图案。
用浮石和水对九颗拔除的第三恒磨牙进行抛光。将每个窝沟作为一个单位进行评估，共得到40个单独的单位，然后将其分为五个治疗组。对每组的咬合面釉质表面进行以下处理：第1组（C）对照组：不进行处理；第2组（P）：抛光并冲洗；第3组（PD）：抛光、冲洗，并用5.25%的次氯酸钠（NaOCl）处理60秒；第4组（PA）：抛光、冲洗，并用37%的HPO酸蚀15秒；第5组（PDA）：抛光、冲洗，用5.25%的次氯酸钠（NaOCl）处理60秒，并用37%的HPO酸蚀15秒。结果显示，第1组（C）（30.18%）和第2组（P）（36.61%）之间存在的有机物质在统计学上无显著差异，但第1组和第2组与第3组（PD）（16.50%）之间存在统计学差异（p<0.002）。在酸蚀组中，第4组（PA）（33.54%）出现了不良的III型图案（稍后讨论），而第5组中仅为7.70%，几乎是第4组的五倍，具有显著的统计学差异（0.05）。当对第4组和第5组的I型和II型蚀刻图案（稍后讨论）进行差异比较时，第4组（PA）获得了26.29%（I型）和1.75%（II型）的蚀刻图案，而第5组（PDA）分别为33.4%（I型）和38.97%（II型）的蚀刻图案。
釉质脱蛋白技术是去除牙齿咬合面有机物质的有效方法，在应用磷酸后，可获得高达72.38%的I型和II型蚀刻图案。I型或II型蚀刻也可通过去除釉质晶体之间的有机物质来确定。
