Phosphorylated threonine as the covalent intermediate in snake venom phosphodiesterase.
作者信息
Culp J S, Butler L G
出版信息
Arch Biochem Biophys. 1986 Apr;246(1):245-9. doi: 10.1016/0003-9861(86)90470-4.
PMID:3008653
Abstract
The covalent intermediate of snake venom phosphodiesterase has been isolated using thymidine 5'-[alpha-32P]triphosphate as substrate. Phosphoamino acid analysis of the labeled enzyme demonstrates that threonine is the active site residue forming the covalent intermediate. 5'-Nucleotide phosphodiesterase is the first enzyme reported to have an active site threonine forming a covalent intermediate.
摘要
Zotero 插件