Food and Feed Quality Unit of Agricultural Product Valorisation Department - Walloon Agricultural Research Centre, Gembloux, Belgium; University of Liège - ULiège, Liège, Belgium.
Food and Feed Quality Unit of Agricultural Product Valorisation Department - Walloon Agricultural Research Centre, Gembloux, Belgium; Catholic University of Louvain - UCL, Louvain-la-Neuve, Belgium.
Talanta. 2018 Nov 1;189:166-173. doi: 10.1016/j.talanta.2018.06.076. Epub 2018 Jun 27.
Fluorescence spectroscopy is a powerful method for protein analysis. Its sensitivity and selectivity allow its use for the detection of blood meal and blood products. This study proposes a novel approach for the detection of hemoglobin in animal feed by synchronous fluorescence spectroscopy (SFS). The objective was to develop a fast and easy method to detect hemoglobin powder and blood meal. Analyses were carried out on standard reference material (hemoglobin and albumin) in order to optimize SFS method conditions for hemoglobin detection. The method was then applied to protein extracts of commercial feed material and compound feed. The results showed that SFS spectra of blood meal and blood products (hemoglobin powder and plasma powder) could be used to characterize hemoglobin. Principal component analysis (PCA) applied to area-normalized SFS spectra of artificially adulterated samples made it possible to define a limit of detection of hemoglobin powder or blood meal of 0.5-1% depending on the feed material. The projection in the PCA graphs of SFS spectra of real commercial compound feeds known to contain or to be free from blood-derived products showed that it was possible to discriminate samples according to the presence of hemoglobin. These results confirmed that SFS is a promising screening method for the detection of hemoglobin in animal feed.
荧光光谱法是一种强大的蛋白质分析方法。其灵敏度和选择性使其可用于检测血粉和血液制品。本研究提出了一种通过同步荧光光谱法（SFS）检测动物饲料中血红蛋白的新方法。目的是开发一种快速简便的方法来检测血红蛋白粉和血粉。对标准参考物质（血红蛋白和白蛋白）进行了分析，以优化 SFS 方法检测血红蛋白的条件。然后将该方法应用于商业饲料和配合饲料的蛋白质提取物。结果表明，血粉和血液制品（血红蛋白粉和血浆粉）的 SFS 光谱可用于表征血红蛋白。基于主成分分析（PCA）对人工掺杂样品归一化面积 SFS 光谱的应用，根据饲料材料的不同，血红蛋白粉或血粉的检测限可定义为 0.5-1%。PCA 图中实际商业配合饲料的 SFS 光谱投影，已知含有或不含血液衍生产品，表明根据血红蛋白的存在可以区分样品。这些结果证实，SFS 是一种有前途的动物饲料中血红蛋白检测的筛选方法。
