Laboratory of Protein Thermodynamics, Departament of Biochemistry and Tissue Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
Electron Microscopy Center, Institute of Biology, State University of Campinas (UNICAMP), Rua Monteiro Lobato, 255, Campinas, SP, 13083-970, Brazil.
Appl Microbiol Biotechnol. 2018 Oct;102(19):8341-8350. doi: 10.1007/s00253-018-9270-4. Epub 2018 Aug 8.
The sterilization of transplant and medical devices should be effective but not detrimental to the structural properties of the materials used. In this study, we examined the effectiveness of chemical and physical agents for inactivating Staphylococcus aureus, a gram-positive bacterium and important cause of infections and biofilm production. The treatment conditions in this work were chosen to facilitate their subsequent use with sensitive materials. The effects of temperature, high hydrostatic pressure, and glutaraldehyde disinfectant on the growth of two strains of S. aureus (ATCC 25923 and BEC 9393) were investigated individually and/or in combinations. A low concentration of glutaraldehyde (0.5 mM), high hydrostatic pressure (300 MPa for 10 min), and moderate temperature (50 °C), when used in combination, significantly potentiated the inactivation of both bacterial strains by > 8 orders of magnitude. Transmission electron microscopy revealed structural damage and changes in area that correlated with the use of pressure in the presence of glutaraldehyde at room temperature in both strains. Biofilm from strain ATCC 25923 was particularly susceptible to inactivation. The conditions used here provided effective sterilization that can be applied to sensitive surgical devices and biomaterials, with negligible damage. The use of this experimental approach to investigate other pathogens could lead to the adoption of this procedure for sterilizing sensitive materials.
移植和医疗器械的灭菌应既有效又不会对所用材料的结构特性产生不利影响。在这项研究中，我们研究了化学和物理因子对金黄色葡萄球菌（一种革兰氏阳性菌，是感染和生物膜形成的重要原因）的灭活效果。本工作中选择了处理条件，以方便其随后用于敏感材料。分别和/或组合考察了温度、高静水压力和戊二醛消毒剂对两种金黄色葡萄球菌（ATCC 25923 和 BEC 9393）生长的影响。低浓度戊二醛（0.5 mM）、高静水压力（300 MPa 持续 10 分钟）和中等温度（50°C）联合使用时，能显著增强两种细菌株的灭活作用，灭活程度超过 8 个对数级。透射电子显微镜显示，结构损伤和面积变化与在室温下使用戊二醛时施加压力有关，两种菌株均出现了这种变化。来自 ATCC 25923 菌株的生物膜尤其容易被灭活。这里使用的条件可对敏感手术器械和生物材料进行有效灭菌，而几乎不会造成损伤。使用这种实验方法来研究其他病原体，可能会促使人们采用这种方法来对敏感材料进行灭菌。
