Olateru-Olagbegi O A, Omoruyi E C, Dada R A, Edem V F, Arinola O G
Immunology Unit, Department of Chemical Pathology, College of Medicine, University of Ibadan and School of Medical Laboratory Science, University College Hospital, Ibadan.
Niger J Physiol Sci. 2018 Jun 30;33(1):51-56.
Pregnancy places a very high demand on physical, physiological and immunological responses of females,especially when aggravated by parasitic infestation. There is strong evidence that maternal infestations with helminth haveprofound effects on immunity to helminths and other pathogens. This case-control study involved 245 pregnant women aged18-40 years (>30 weeks of gestation) recruited from three secondary level hospitals in Ibadan, Nigeria. Morning stoolsamples collected from pregnant women were examine for intestinal helminths using formol-ether concentration method. Atotal of 38 participants comprising 17 Helminth Positive (HP) and 21 Helminth Negative (HN) pregnant women werepurposely selected for the study. Sera from these women (38) and their babies' cord (38) were analysed for immune factors[interleukins 6 and 8 (IL-6, IL-8), tumor necrosis factor-alpha (TNF-α) and immunoglobulin E (IgE)] were analyzed usingELISA. Anthropometric indices [weight and height in mothers and babies and Chest Circumference (CC) in babies] weremeasured using standard methods. Data were subjected to descriptive statistics and analysed using Student t-test and Pearsoncorrelation at α0.05. Only Ascaris lumbricoides was found in the 17 (6.9%) infested pregnant women. The mean levels of IL-6 (57.8 ± 32.8 vs 52.8±39.6 pg/mL), IL-8 (24.3±3.5 vs 22.0±7.1 pg/mL) and IgE (333.3±96.6 vs 242.3±96.8 IU/mL) weresimilar in HP when compared with HN. In cord sera, IL-8 level was significantly higher in babies of HP (23.7±3.9 pg/mL)compared with babies of HN (20.1±5.9 pg/mL). The levels of IL-6, TNF-α and nutritional indices in HP had significantpositive correlation with corresponding levels in babies of HP mothers. Only CC was significantly lowered in babies of HPcompared with HN mothers. Other anthropometric indices were not significantly different. Therefore, this present studysuggests that helminth infestation may lead to strong Th2 immune responses as is reflected by the cytokine levels of mothersand babies as well as anthropometric measurements of babies of infested mothers. The outcomes of this study provide basisto deworm pregnant women during pregnancy.
怀孕对女性的身体、生理和免疫反应提出了很高的要求，尤其是在寄生虫感染加剧的情况下。有强有力的证据表明，孕妇感染蠕虫对其对蠕虫和其他病原体的免疫力有深远影响。这项病例对照研究涉及从尼日利亚伊巴丹的三家二级医院招募的245名年龄在18 - 40岁（妊娠>30周）的孕妇。采用甲醛-乙醚浓缩法对孕妇清晨采集的粪便样本进行肠道蠕虫检测。总共特意挑选了38名参与者，包括17名蠕虫阳性（HP）和21名蠕虫阴性（HN）孕妇进行研究。使用酶联免疫吸附测定法（ELISA）分析这些女性（38名）及其婴儿脐带血（38份）中的免疫因子[白细胞介素6和8（IL - 6、IL - 8）、肿瘤坏死因子-α（TNF - α）和免疫球蛋白E（IgE）]。使用标准方法测量人体测量指标[母亲和婴儿的体重和身高以及婴儿的胸围（CC）]。数据进行描述性统计，并使用学生t检验和Pearson相关性分析，α为0.05。在17名（6.9%）受感染的孕妇中仅发现蛔虫。与HN相比，HP中IL - 6（57.8±32.8对52.8±39.6 pg/mL）、IL - 8（24.3±3.5对22.0±7.1 pg/mL）和IgE（333.3±96.6对242.3±96.8 IU/mL）的平均水平相似。在脐带血血清中，HP婴儿的IL - 8水平（23.7±3.9 pg/mL）显著高于HN婴儿（20.1±5.9 pg/mL）。HP中的IL - 6、TNF - α水平和营养指标与HP母亲的婴儿中的相应水平呈显著正相关。与HN母亲的婴儿相比，HP母亲的婴儿中只有CC显著降低。其他人体测量指标无显著差异。因此，本研究表明，蠕虫感染可能导致强烈的Th2免疫反应，这在母亲和婴儿的细胞因子水平以及受感染母亲的婴儿的人体测量中得到体现。本研究结果为孕期给孕妇驱虫提供了依据。
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