Dong Sufang, Wu Lixian, Long Fuquan, Wu Qiang, Liu Xiang, Pei Hua, Xu Ke, Lu Yajun, Wang Ying, Lin Yingzi, Xia Qianfeng
Laboratory of Tropical Biomedicine and Biotechnology, School of Tropical Medicine and Laboratory Medicine, Hainan Medical University, Haikou 571199, China.
Department of STD, Shanghai Dermatology Hospital, Shanghai 200443, China.
Acta Trop. 2018 Nov;187:165-168. doi: 10.1016/j.actatropica.2018.08.007. Epub 2018 Aug 7.
Melioidosis is an infectious disease caused by Burkholderia pseudomallei, mainly found in Southeast Asia and northern Australia. In Hainan, sporadic cases were first described in 1990; since then, more cases have been identified. No systematic study has yet been done to detect the environmental source of the organism and its true extent in Hainan. This study is aimed to confirm the prevalence of B. pseudomallei in soil samples in Hainan. 1080 soil samples from 18 different counties were collected from 3 sampling points of 360 sites. They were screened for the presence of B. pseudomallei by Ashdown selective media. Suspected colonies of B. pseudomallei were confirmed by biochemical test and a specific PCR assay. 48 of 360 sites (13.3%) were positive for B. pseudomallei, including all coastal counties in Hainan Island. This study revealed the prevalence and distribution of B. pseudomallei in the soil environment in Hainan Island of southern China and may be helpful to understand the distribution of B. pseudomallei and to access its epidemiological importance.
类鼻疽是由类鼻疽伯克霍尔德菌引起的一种传染病，主要见于东南亚和澳大利亚北部。在海南，1990年首次报道了散发病例；此后，又发现了更多病例。目前尚未对海南该病原体的环境来源及其真实分布范围进行系统研究。本研究旨在确认海南土壤样本中类鼻疽伯克霍尔德菌的流行情况。从360个采样点的3个采样点收集了来自18个不同县的1080份土壤样本。通过阿什当选择性培养基筛选类鼻疽伯克霍尔德菌的存在。通过生化试验和特异性PCR检测确认疑似类鼻疽伯克霍尔德菌菌落。360个采样点中有48个（13.3%）类鼻疽伯克霍尔德菌呈阳性，包括海南岛所有沿海县。本研究揭示了中国南方海南岛土壤环境中类鼻疽伯克霍尔德菌的流行情况和分布，可能有助于了解类鼻疽伯克霍尔德菌的分布并评估其流行病学重要性。
